PEDF对人肾小球系膜细胞VEGF表达及活性氧簇产生的影响

观察不同浓度高糖培养下及人重组色素上皮衍生因子(PEDF)干预后人肾小球系膜细胞活性氧簇水平、血管内皮生长因子(VEGF)mRNA和蛋白的改变.结果 显示活性氧簇水平、VEGF mRNA和蛋白的表达随糖浓度的升高而增加;PEDF干预后,高糖环境下活性氧簇水平及VEGF的表达明显下降,且呈浓度依赖性.提示PEDF可能通过改善血管通透性和抑制氧化应激延缓糖尿病肾病的进展.

Effects of pigment epithelium-derived factor on the expression of vascular endothelial growth factor and reactive oxygen species in the human mesangial cells

To investigate the expression of vascular endothelial growth factor (VEGF)and the levels of reactive oxygen species (ROS)in human mesangial cells cultured in high glucose and being intervened by pigment epithelium-derived factor (PEDF). The results showed that with increased glucose concentration, the expressions of VEGF and ROS generation also gradually increased and PEDF significantly downregulated VEGF expression and ROS generation in a dose-dependent manner. PEDF may delay the diabetic nephropathy progress by improving vascular permeability and inhibiting oxidative stress.