原发性肝细胞癌Survivin,CyclinD1的表达及其临床意义

目的：探讨Survivin和CyclinD1在原发性肝细胞癌（HCC）组织中的表达及两者在肝癌发病机制中的作用和临床意义.方法：利用免疫组织化学SP法检测50例原发性肝癌组织、20例正常肝组织中Survivin和CyclinD1蛋白表达情况.结果：Survivin蛋白在肝癌中的阳性表达率为66.0%（33/50）,显著高于正常肝组织组（均为阳性表达）（P＜0.001）,阳性表达与肝癌的肝内转移和肝癌的多发性有关（P=0.019,P=0.030）.CyclinD1蛋白在肝癌中的阳性率为44.0%（22/50）,显著高于正常肝组织组（均为阴性表达）（P＜0.001）,阳性表达与肝癌的肝内转移和肝癌的分化程度有关（P=0.035,P=0.036）.Survivin的阳性表达与CyclinD1的阳性表达呈正相关（r=0.466,P＜0.001）.Survivin和CyclinD1的过表达均与患者术后＜3年生存期相关（P=0.018,P=0.013）.结论：Survivin与CyclinD1在肝细胞癌的发生及发展过程中起着不同程度的作用,联合检测Survivin与CyclinD1对判定原发性肝细胞癌预后可提供一定的依据.

Expression and clinical significance of survivin and cyclin D1 protetin in primary hepatocellular carcinoma

Objective:To investgate the expression and the clinical significance of Survivin and CyclinD1 with the possible mechanism in the primary hepatocellular carcinoma(HCC) .Methods:SP immunohistochemical staining was performed to detect the expression of Survivin and CyclinD1 protein in 50 HCC tissues and 20 normal liver tissues.Results:The positive rate of Survivin expression in HCC were 66.0%(33/50) ,which was significantly higher than that in normol liver tissues(negative expression) (P<0.00l) ,the expression of Survivin was correlated with intrahepatic dissemination and multiple masses(P=0.019,P=0.030) ,The positive rate of CyclinD1 expression in HCC was 44.0%(22/50) ,which was significantly higher than that in normol liver tissues(negative expression) (P<0.001) ,the expression of CyclinD1 was correlated with intrahepatic dissemination and tumor grade(P=0.035,P=0.036) .There was a positive correlation between Survivin expression and CyclinD1 expression(r=0.466,P<0.001) .The high Survivin and CyclinDl expression was associated with the survival stage less than three years(P=0.018,P=0.013) .Conclusions:The experiment suggests that Survivin and CyclinDi may play certain roles in the oncogenesis and progression of HCC,but to diffrent extent,joint detection of the Survivin and CyclinDl gene products can supply certain evidence on prognosis of primary hepatocellular carcinoma.