先天性肛门直肠畸形模型大鼠肛门内括约肌肌间神经节细胞及神经丛的病理改变

目的观察肛门直肠畸形动物模型肛门内括约肌肌间神经节细胞及神经丛的数目和形态。方法用乙烯硫脲诱导健康妊娠Wistar-Imammichi大白鼠产生有内括约肌的肛门直肠畸形胎鼠,取胎仔行正中矢状面连续切片,经染色后观察直肠肛管发育情况及肛门内括约肌肌间神经节细胞和神经丛数的数目。结果实验组肛门直肠畸形胎鼠每个视野的肛门内括约肌肌间神经节细胞数为(1.206±0.012),神经丛数仅为(0.308±0.051),对照组正常胎鼠分别为(3.710±0.043)和(1.227±0.072),两者比较差异均有统计学意义(P<0.05～0.01),而实验组正常胎鼠两项数值分别为(3.012±0.032)和(1.187±0.004),与对照组相比,差异无统计学意义。结论肛门内括约肌肌间神经节细胞数和神经丛数的减少是肛门直肠畸形的重要病理改变。

Pathologic Changes of Ganglion Cell and Nerve Plexus in Internal Anus Sphincter of Rats with Congenital Anorectal Malformations

Objective To observe the pathologic changes of ganglion cell and nerve plexus in internal anus sphincter of rats with congenital anorectal malformations (ARM).Methods Healthy and pregnant Wistar-Imammichi rats were induced to ARM by ethylene thiourea (ETU). There were 67 rats with ARM at all. The pelvis was continuously cut from the exactly midsagittal plane. The HE staining was performed, and the numbers of the ganglion cell and nerve plexus in the internal anus sphincter were observed under the microscope.Results The average numbers of the ganglion cell and nerve pluxes in the ARM embryos were (1.206±0.012) and (0.308±0.051). Those of the normal control embryos were (3.710±0.043) and (1.227±0.072) respectively. There was a significant difference between two groups ( P<0.05～0.01). While, those of the embryos without ARM which dealt with ETU were (3.012±0.032) and (1.187±0.004) respectively, didn't differ from the normal control embryos.Conclusion The decrease of the ganglion cell and the nerve plexus in internal anus sphincter is an important pathologic change of ARM.