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| 转染外源性白细胞介素6基因增强乳腺癌细胞株MCF-7表达肿瘤相关抗原 | |
| 引用本文： | 陈方正,江贤鹏,陈淼,Jonathan FH,Robert LE,姜希凌,李欣燕. 转染外源性白细胞介素6基因增强乳腺癌细胞株MCF-7表达肿瘤相关抗原[J]. 中国临床药理学与治疗学, 2004, 9(12): 1408-1410 |
| 作者姓名： | 陈方正 江贤鹏 陈淼 Jonathan FH Robert LE 姜希凌 李欣燕 |
| 作者单位： | 1. 江苏省中西医结合医院急诊外科,南京,210000,江苏 2. The Elliott Breast Research and Treatment Center, Baton Rouge, LA 70816, USA 3. 中国药科大学江苏省药代动力学重点实验室,南京,210000,江苏 |
| 摘 要： | 目的 :探索白细胞介素 6 (interleukin 6 ,IL 6 )增强乳腺癌细胞表达乳腺癌抗原 (CA15 3)和癌胚抗原 (CEA)的作用。方法 :将含IL 6蛋白编码顺序1176bpcDNA插入Pci neo哺乳动物表达载体。将重组载体转染MCF 7乳腺癌细胞 ,采用ELASA方法测定细胞培养上清液内IL 6浓度 ,用MEIA(microp articalenzymeimmunoassay)方法测定上清液中肿瘤相关抗原CA15 3、CEA和CA12 5。结果 :含有外源IL 6基因的MCF 7细胞分泌IL 6浓度 (338.5±2 2 .6pg·10 -6细胞 )明显高于父本没有含外源基因的MCF 7细胞 (2 5 .4± 4 .6pg·10 -6细胞 )和仅含空载体Pci neo的MCF 7细胞 (19.6± 3.0pg·10 -6细胞 ) (P<0 .0 1)。细胞培养d 3后 ,带外源IL 6基因的MCF 7细胞培养上清液中CA15 3和CA12 5水平 (分别为 14 .9± 2 .3和 38.8± 5 .1μg·10 -6细胞 )明显高于父本 (分别为 6 .6± 1.5和 10 .0± 1.6 μg·10 -6细胞 )和空载体Pci neo的MCF 7细胞 (分别为 3.4±0 .7和 14 .6± 2 .2 μg·10 -6细胞 ,P <0 .0 5 )。而转染IL 6基因没有明显改变CEA表达 (P >0 .0 5 )。结论 :IL 6具有诱导肿瘤相关抗原的表达和增强肿瘤细胞的免疫原性的作用 ,提示IL 6可能增强机体对肿瘤的免疫反应性。 |
| 关 键 词： | 白细胞介素6 乳腺癌细胞 乳腺癌抗原 癌胚抗原 |
| 文章编号： | 1009-2501(2004)12-1408-03 |
| 修稿时间： | 2004-01-06 |
Increased expression of tumor-associated antigen after interleukin 6(IL-6) mediated transfection in breast cancer cells |
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| Chen Fang-Zheng,Jiang Xian-Peng,Duan Jin-Ao,Chen Miao,Jonathan FH ,Robert LE,Jiang Xi-Ling,Li Xin-Yan Jiangsu Provincial Hospital of Integrated Traditional Chinese and Western Medici ne,Nanjing ,Jiangsu,China, the Elliott Breast Research and Treatme nt Center,Baton Rouge,LA ,USA, Key Laboratory of Pharmacokinetics,China Pharmaceutical University,Nanjing ,Jiangsu,China WT HZ][. Increased expression of tumor-associated antigen after interleukin 6(IL-6) mediated transfection in breast cancer cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(12): 1408-1410 | |
| Authors: | Chen Fang-Zheng Jiang Xian-Peng Duan Jin-Ao Chen Miao Jonathan FH Robert LE Jiang Xi-Ling Li Xin-Yan Jiangsu Provincial Hospital of Integrated Traditional Chinese Western Medici ne Nanjing Jiangsu China the Elliott Breast Research Treatme nt Center Baton Rouge LA USA Key Laboratory of Pharmacokinetics China Pharmaceutical University Nanjing Jiangsu China WT HZ][ |
| Affiliation: | Chen Fang-Zheng,Jiang Xian-Peng1,Duan Jin-Ao,Chen Miao2,Jonathan FH 1,Robert LE1,Jiang Xi-Ling2,Li Xin-Yan2 Jiangsu Provincial Hospital of Integrated Traditional Chinese and Western Medici ne,Nanjing 210000,Jiangsu,China, 1 the Elliott Breast Research and Treatme nt Center,Baton Rouge,LA 70816,USA, 2 Key Laboratory of Pharmacokinetics,China Pharmaceutical University,Nanjing 210000,Jiangsu,China WT HZ][ |
| Abstract: | |
| Keywords: | interleukin 6 breast cancer cells breast cancer antigen ca rcinoembryonic antigen |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |