甲状腺滤泡癌组织P—Akt与PTEN表达及其与血管生成关系

目的探讨磷酸化蛋白激酶B（P—Akt）、磷酸酯酶基因（PTEN）异常表达与甲状腺滤泡癌血管生成之间的关系。方法采用免疫组织化学PV-6000两步法检测甲状腺滤泡癌、甲状腺腺瘤及结节性甲状腺肿标本中P—Akt、PTEN、血管内皮生长因子（VEGF）及微血管密度（MVD）的表达。结果P—Akt在甲状腺滤泡癌组织中的表达较非甲状腺滤泡癌组织显著增高（χ^2=43．45，P〈0．001）。PTEN蛋白在甲状腺滤泡癌组织中的表达显著降低。甲状腺滤泡癌组织中VEGF表达与非甲状腺滤泡癌组织比较差异有显著性（χ^2=20．00，P〈0．01）。甲状腺滤泡癌组织中P—Akt表达与VEGF表达具有相关性（P=0．04）。结论P—Akt高表达在甲状腺滤泡癌发生发展及继发转移过程中可能发挥重要作用。甲状腺滤泡癌组织中PTEN的失表达可能通过调控P—Akt促进肿瘤的侵袭与转移。VEGF在甲状腺滤泡癌的生长和转移中占重要地位，甲状腺滤泡癌中可能存在Akt／VEGF通路，Akt可能是调控VEGF表达通路中的关键因子。

RELATIONSHIP AMONG THE EXPRESSIONS OF P-Akt, PTEN, AND ANGIOGENESIS IN FOLLICULAR THYROID CARCINOMA

Objective To investigate the relationship among the abnormal expressions of P-Akt, PTEN, and angiogenesis in human follicular thyroid carcinoma （FTC）. Methods Using immunohistochemical PV-6000 method, the abnormal expressions of P-Akt, PTEN, vascular endothelial growth factor （VEGF） and microvessel density （MVD） in specimens of FTC and goiter were detected. The association of abnormal expressions of P-Akt and PTEN in FTC were detected. Results The expression of P-Akt in FTC was significantly higher than that in non-FTC tissue （χ^2 =43.45 ,P〈0.01）. The expression of PTEN in FTC was obviously decreased. The difference in VEGF expression between FTC and non-FTC tissues was significant （χ^2= 20. 00, P〈 0.01 ）. The correlation between the expressions of P Akt and VEGF in FTC was statistically significant （P=0.04）. Conclusion The overexpression of P Akt might play an important role in the genesis and metastasis of FTC. The loss of PTEN expression might promote the invasion and metastasis of FTC through regulating the expression of P Akt. VEGF is also an important factor in the genesis and metastasis of FTC. There might be an Akt/VEGF pathway in FTC, in which Akt regulates the expression of VEGF.