过敏性紫癜病儿急性期免疫细胞功能变化 |
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引用本文: | 柏翠,宗金宝,张秋业.过敏性紫癜病儿急性期免疫细胞功能变化[J].康复与疗养杂志,2010(2):137-139. |
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作者姓名: | 柏翠 宗金宝 张秋业 |
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作者单位: | [1]青岛大学医学院附属医院儿科,山东青岛266003 [2]青岛大学医学院附属医院免疫检验科,山东青岛266003 |
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摘 要: | 目的探讨过敏性紫癜(HSP)病儿急性期免疫细胞功能变化及其意义。方法以53例HSP急性期病儿为研究对象,并根据有无尿检异常分为。肾炎(HSPN)组和非肾炎(NHSPN)组,以30例正常儿童作为对照组。采用流式细胞术检测3组外周血CD3^+细胞、CD4^+细胞、CD8^+细胞、CD4^+/CD8^+比值、CD4^+CD25^+细胞、NK细胞(CD16^+CD56^+)和B淋巴细胞(CD19^+)水平。结果HSPN组和NHSPN组外周血CD3^+细胞、CD4^+细胞、CD4^+/CD8’比值、CD4^+CD25^+细胞和NK细胞水平较对照组显著降低,CD8^+细胞和B淋巴细胞水平较对照组显著增高(F=6.80~46.40,q=2.81~4.25,P〈0.01、0.05),而HSPN组和NHSPN组间比较差异无统计学意义(P〉0.05)。HSP病儿CD4^+CD25^+细胞水平与CD4^+细胞、CD4^+/CD8^+比值呈正相关(r=0.369、0.285,P〈0.01、0.05),与CD19^+细胞呈负相关(r=-0.279,P〈0.05),而与CD8^+细胞和CD16^+CD56^+细胞无相关性(r=0.009、-0.104,P〉0.05)。结论HSP病儿急性期存在免疫细胞功能异常,T细胞亚群紊乱,CD4^+CD25^+T细胞和NK细胞数量降低,导致B细胞呈多克隆活化,在HSP发病机制中具有重要作用。
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关 键 词: | 紫癜 过敏性 儿童 免疫 细胞 流式细胞术 |
FUNCTIONAL CHANGES OF IMMUNE CELLS IN CHILDREN WITH HENOCH-SCHONLEIN PURPURA IN ACUTE STAGE |
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Authors: | BAI CUI ZONG JIN-BAO ZHANG QIU-YE |
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Institution: | (Department of Pediatrics, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China) |
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Abstract: | Objective To investigate the functional changes of immune cells in children with Henoch-Schonlein Purpura (HSP) in acute stage and their significance. Methods Fifty-three children with HSP were involved in this study, who were divided into Henoch-Schonlein Purpura-nephritis (HSPN) group and non-HSPN (NHSPN) based on whether the urine analysis was normal or not. Thirty healthy children were served as control. Flow cytometry was applied to detect the amounts of CD3^+ cells, CD4^+ cells and CD8^+ cells, CD4^+/CD8^+ ratio, and the levels of CD4^+ CD25^+ cells, NK cells (CD16^+ CD56^+) and B cells (CD19^+ ) in peripheral blood. Results The amounts of CD3^+ cells, CD4^+ cells, CD4^+ CD25^+ cells, NK ceils and the ratio of CD4^+/CD8^+ in the HSP and NHSPN children were significantly lower than those in the control children, while CD8^+ cells and B cells were significantly higher (F=6. 80-46. 40 ;q=2.81-4.25 ;P〈0. 01,0. 05). The differences between those in HSP and the NHSPN children were not significant (P〉0.05). The amount of CD4^+ CD25^+ cells of the HSP was positively correlated with that of CD4^+ cells and CD4^+/CD8^+ ratio (r= 0. 369, P〈0.01 ; r= 0. 285, P〈0.05), and negatively correlated with tthat of CD19^+ cells (r= -0. 279 ,P〈O. 05), but not correlated with those of CD8^+ cells and CD16^+ CD56^+ cells (r=0. 009,-0. 104;P〉0.05). Conclusion Children with HSP in acute stage have a disordered cell-mediated immunity, which mainly manifests as derangement of T-cell subsets, decrease of CD4^+CD25^+ T cells and NK cells, resulting in polyclonal B-cell activities, which plays an important role in the pathogenesis of this condition. |
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Keywords: | purpura Schoenlein-Henoch child immunity cell flow cytometry |
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