稀土微肥对铁皮石斛试管苗壮苗的影响

目的研究稀土微肥对铁皮石斛试管苗壮苗的影响。方法通过正交试验,以石斛小苗为外植体,培养在附加了3种不同稀土元素组合的1/2MS 20%的香蕉提取物的培养基上进行培养。结果3种稀土元素中的镧(La)对试管苗增质量、叶绿素b、总叶绿素和石斛多糖的量都有显著的影响;3种稀土元素都对试管苗的叶片数、根数、分蘖数以及叶绿素a的量影响不显著。结论为铁皮石斛试管苗的壮苗以及品质的提高提供了科学的依据。

Effects of rare-earth element addition on seedling growth of Dendrobium candidum

Objective To examine the effects of rare-earth elements on the seedling growth of Dendro-bium candidum. Methods The seedlings were tested as the explants and cultured on 1/2 MS culture medium with extract from banana at the concentration of 20% and different portions of three kinds of rare earth elements. Results Lanthanum (La), in the three rara-earth elements, has a remarkable effect on the seedlings including increment weight, chlorophyll b, total chlorophyll, and content of Dendrobium polysaccharide. But all of the three rara-earth elements have not remarkable effects on the following aspects, such as the differentiation number of leaves, root, divarication, and chlorophyll a content. Conclusion The above mentioned method provides a scientific way to the seedling growth of D. candidum and improvement of the quality.