中药狼疮方对狼疮样BXSB小鼠脾细胞CD134/CD134L表达的影响

目的:探讨CD134/CD134L共刺激信号在SLE发病机制中的可能作用及狼疮方对其表达的影响.方法:采用狼疮样BXSB小鼠模型,随机分为3组:狼疮方治疗组、泼尼松治疗组、空白模型组,每组6只,疗程10周.另设与BXSB小鼠同基因的正常C57BL/6小鼠6只为正常对照组.分别采集上述各组小鼠外周血和脾组织进行检测.结果:(1)与正常对照组比较,模型组BXSB小鼠的血清IgG和抗ds-DNA抗体水平和脾组织CD4 CD134 、CD8 CD134 、CD19 CD134L 、CD23 CD134L 的百分比及CD134、CD134L的mRNA拷贝数都显著增高(P＜0.05或P＜0.01).(2)狼疮方治疗组或泼尼松治疗组的血清IgG、抗ds-DNA抗体水平和脾组织CD4 CD134 、CD8 CD134 、CD19 CD134L 、CD23 CD134L 的百分比及CD134、CD134L的mRNA拷贝数都显著低于模型组(P＜0.05或P＜0.01),而与正常对照组比较无统计学差异(P均＞0.05).结论:狼疮样BXSB小鼠存在CD134/CD134L的异常表达.狼疮方可减轻狼疮样小鼠高丙种球蛋白血症,减少体内自身抗体产生,对CD134/CD134L存在泼尼松相当的下调作用,抑制T、B细胞的异常活化,有效地治疗SLE.

Effect of Lupus Recipe on Gene Expression and Protein Synthesis of CD134 and CD134L in Spleen Cells of Lupus-prone BXSB Mice

Objective:To investigate the effects of lupus recipe on gene expression and protein synthesis of CD_(134) and CD_(134)L in splenic cells of lupus-prone BXSB mice.Methods:Eighteen male lupus-prone BXSB mice model were used in the experiment.The model mice were divided into three groups: un-treated model group,lupus recipe(LR)-treated group,and prednisone treated group.Model mice were killed in 10 weeks.The control groups consisted of 6 syngenetic normal C57BL/6 male mice.Peripheral blood and spleen tissues were collected for the study.Results:(1)the serum levels of total IgG and anti-dsDNA antibody in un-treated model group were higher than that in other groups.And there were no differences among LR-treated group,prednisone-treated group and control group.(2)The positive cell percentages of CD_4~+ CD_(134)~+,CD_8~+ CD_(134)~+,CD_(19)~+ CD_(134)L~+,CD_(23)~+ CD_(134)L~+ and the CD_(134),CD_(134) mRNA expressions from un-treated model group were significantly higher than those from control groups(P<0.05,P<0.01).(3)The LR or prednisone significantly reduced the positive cell percentages of CD_4~+ CD_(134)~+,CD_8~+ CD_(134)~+,CD_(19)~+ CD_(134)L~+,CD_(23)~+ CD_(134)L~+ and the CD_(134),CD_(134)L mRNA expressions in LR-treated group or prednisone-treated group(P<0.05,P<0.01 comparing with model group),while the difference between the treated group and the normal group was not significant.Conclusion:BXSB mice has abnormal expression of CD_(134) and CD_(134)L.Lupus recipe reduces the serum levels of IgG and auto-antibody production and can treat systemic lupus erythematosus effectively.