TPPA在梅毒临床血清学诊断和室间质评中的应用

目的评价TRUST和TPPA在梅毒血清学诊断和室间质评中的价值。方法采用TRUST和TPPA检测80例梅毒患者和67例非梅毒患者血清；1998～1999年采用TRUST检测室间质评样本，从2000年开始采用TPPA检测室间质评样本。结果梅毒患者TRUST和TPPA阳性率分别为86.3%(69/80)与100%；TRUST在室间质评样本中的敏感性44.44%，特异性90.00%，符合率68.75%，而TPPA的敏感性、特异性、符合率达到100%。两者的敏感性存在明显差别(χ2＝9.35，P＜0.01)。结论TPPA具有很高的敏感性、特异性，可作为临床诊断梅毒的首选方法。

Application of Treponema Pallidun Particle Agglutination(TPPA)Test in Clinical Serodiagnos of Syphilis and External Quality Assessment

Objective　To evaluate the value of Tolidime Red Unheated Serum Test (TRUST) and External Quality Assessment.Methods　To test the serums of syphilis and non-syphilis by TRUST and TPPA.The samples of External Quality Assessment from 1998 to 1999 year and 2000 year were tested by TRUST and TPPA respectively.Results　The positive rates of syphilis serum tested by TRUST and TPPA were 86.3%(69/80) and 100%.The sensitivity and specificity of TRUST were 44.44% and 90.00% in External Quality Assessment.The accuracy was 68.75%.The sensitivity and specificity and accuracy of TPPA were all 100%.There was an evident difference between their sensitivties(χ2＝9.35，P＜0.01).Conclusions　TPPA has very high sensitivity and specificity.It can be first selected clinical method to diagnostic Syphilis.