侧脑室注射苯并（a）芘致大鼠脑组织形态学及行为学的改变

目的]研究侧脑室注射苯并（a）芘B（a）P]致大鼠脑组织形态学及行为学的改变。方法]32只SD大鼠随机分为4组，每组8只，染毒组分为高、中、低3个剂量组（31．5、63．1、126．2μg/kg体重），并设橄榄油溶剂对照组。采用侧脑室微量注射染毒处理，每周1次，连续3周；对照组用橄榄油溶剂作平行处理。记录一般情况及神经系统表现；实验第6周末采用跳台实验法测大鼠的学习记忆成绩，并取各组大鼠脑组织大脑皮质、海马、小脑作病理切片观察组织损伤的形态学指标。结果]与对照组相比，低剂量组潜伏期缩短（P〈0．05）；中剂量组学习期和记忆期的错误次数即EN1和EN2均增加（P〈0．01）、潜伏期缩短（P〈0．05）；高剂量组EN1增加（P〈0．01）、EN2增加（P〈0．05）、潜伏期缩短（P〈0．01）；染毒组在光镜下可见到组织损伤的形态学改变，高剂量组各组织在光镜下可见较为集中的坏死区，而对照组的组织结构未见异常。结论]病理形态学指标及跳台实验结果显示B（a）P具有神经毒性，可损伤大鼠脑组织并引起神经行为的改变。

Study on Morphology in Rat Brain Tissue and Behavioral Change by Intracerebroventricular Injecting Benzo(a)pyrene

Objective]To stduy the morphology in rat brain tissue and behavioral changes by intracerebroventricular injecting Benzo(a)pyrene.Methods] 32 SD rats were divided into 4 groups at random,i.e.3 exposed groups and a vehicle control group. The exposed groups were treated by lateral cerebral ventricle micro-injection with B(a)P dissolved in olive oil at 31.5,63.1 and 126.2 mg/kg respectively,once every week for 3 weeks,the vehicle control group was treated with olive oil. The general conditions and signs of ne...