沙利度胺对A549细胞体外增殖的影响及其作用机制

目的探讨沙利度胺对人非小细胞肺癌细胞株A549体外增殖和其对A549细胞中血管内皮生长因子(VEGF)和血管内皮生长因子C(VEGF-C)表达的影响。方法体外培养A549细胞株,分为对照组和沙利度胺组,后者分别加入不同质量浓度(5,10,20,40,60 mg/L)的沙利度胺体外培养24 h。采用MTT法检测对A549细胞增殖的抑制率,采用RT-PCR和Western-blot方法分别检测各组VEGF和VEGF-C mRNA和蛋白水平的表达。结果沙利度胺体外能抑制A549细胞增殖,呈浓度依赖性(P<0.05)。不同浓度沙利度胺作用于A549细胞后,VEGF和VEGF-C mRNA和蛋白水平的表达均降低(P<0.05)。结论沙利度胺体外能抑制A549细胞的增殖,呈浓度依赖性,其作用机制可能与沙利度胺下调VEGF及VEGF-C的表达有关。

Study on effects of thalidomide on proliferation of A549 cells in vitro and its mechanism

Purpose To investigate the effects of thalidomide on proliferation in vitro of human non small-cell lung cancer A549 cells,and to detect the effects on the expressions of VEGF and VEGF-C of A549 cells.Methods Culture the A549 cells in vitro and divide into two groups:control group and thalidomide group which used different mass concentrations(5,10,20,40,60 mg/L) thalidomide interfere for 24 h in the A549 cells.The cell growth inhibition was assessed by MTT-microculture tetrazolium,the expression of VEGF and VEGF-C mRNA and protein level were detected respectively by RT-PCR and Western-blot.Results Thalidomide can inhibit the growth of A549 cells in vitro in dose-dependent manner(P<0.05).After different mass concentrations of thalidomide interfere for 24 h in the A549 cells,the mRNA and protein level expressions of VEGF and VEGF-C all decreased(P<0.05).Conclusion Thalidomide can inhibit the growth of A549 cells in vitro in dose-dependent manner,mechanism of which might relate to the suppression of the expressions of VEGF and VEGF-C.