无毒棉籽水提物对皮质酮诱导的PC12细胞损伤的对抗作用

目的 :探讨无毒棉籽水提物 (CTN-W)抗抑郁、抗焦虑作用的可能机理。方法与结果 :提取大鼠大脑皮层突触膜并与CTN-W 0.01,0.03,0.10,0.30mg·mL^-1直接孵育 ,放免法测定腺苷酸环化酶 (AC)活性的改变 ,发现CTN-W可剂量依赖地激活AC ;MTT比色法研究表明 ,CTN-W 0.08,0 .40,2.00mg·mL^-1与皮质酮 2×10-4mol·L^-1共孵PC12细胞 48h后可防止皮质酮所致的PC12神经细胞损伤。结论 :CTN-W的作用可能与信号转导系统AC cAMP通路的激活 ,从而对损伤神经元产生保护作用有关 ,此二者共同组成了CTN-W的部分作用机制。

Antagonistic Effect of Aqueous Extract of Detoxified Cottonseeds on Corticosterone-induced Lesion in Cultured PC12 Cells

Objective:To study the possible mechanism of aqueous extract of detoxified cottonseeds (CTN-W). Method and result:CTN-W 0.01,0.03,0.10,0.30 mg·mL^-1 was incubated directly with the synaptic membrane extracted from the cerebral cortex in rats, and adenylyl cyclase (AC)activity was detected by using radio immunoassay. Result: Showed that CTN-W could activate AC in a dose dependend manner.After incubation with PC12 cells in the presence of corticosterone 2×10^-4 mol·L^-1 for 48h,CTN-W 0.08,0.4,2 mg·mL^-1 protected PC12 cells from the lesion induced by corticosterone. Conclusion:Antidepressant and anxiolytic effects of CTN-W are related with the activation of AC cAMP pathway in signal transduction system, thus protecting neurons from the lesion.These two aspects maybe partly form the mechanism of CTN W's action.