| 预防血管成形术后再狭窄的C-sis反义寡脱氧核苷酸在血清中的稳定性 |
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| 引用本文： | 杨敏,唐其东,蔡立丰,林曙光. 预防血管成形术后再狭窄的C-sis反义寡脱氧核苷酸在血清中的稳定性[J]. 岭南心血管病杂志, 1999, 5(3): 208-209 |
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| 作者姓名： | 杨敏 唐其东 蔡立丰 林曙光 |
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| 作者单位： | 1. 广东省心血管病研究所临床药理室,广州,510080
2. 中山大学生命科学院药学系 |
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| 摘 要： | 目的 探讨自行设计合成的C-sis癌基因反义寡脱氧核苷酸(AODN)和C-sis癌基因反义硫代磷酸寡脱氧核苷酸(PS-AODN)血清稳定性。方法 用小牛血清模拟体内条件于不同时相点进行稳定性试验,用高效液相色谱法测定血清中AODN和FS-ODN。结果 AODN在血清中1小时内降解43.3％,1天降解53.1％。PS-ODN 1 h降解23.7％,第6天降解59.6％。PS-AODN血清中的稳定性明显高于AODN。结论 PS-AODN在血清中有较好的稳定性,提示其具有潜在的临床应用价值。
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| 关 键 词： | 反义寡脱氧核苷酸 硫代磷酸反义寡脱氧核苷酸 稳定性 C-sis原癌基因 |
The Stabilization of C-sis Proto-oncogene Phosphorthioate Antisense Oligodeoxynucleotides Prevented Restenosis in Serum |
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| Yang Min,Tang Qidong,Cai Lifeng,Lin Shuguang. The Stabilization of C-sis Proto-oncogene Phosphorthioate Antisense Oligodeoxynucleotides Prevented Restenosis in Serum[J]. South China Journal of Cardiovascular Diseases, 1999, 5(3): 208-209 |
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| Authors: | Yang Min Tang Qidong Cai Lifeng Lin Shuguang |
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| Abstract: | ve To investigate stability of the self - designed and synthesized C-sis phosphorthioate antisense oligodeoxynucleotides (PS - AODN) and C-sis proto - oncogene antisense oligodeoxynucleotides (AODN) in serum. Methods The stability of C-sis PS-AODN and C-sis AODN in serum was tested in different timepoints with HPLC method.Results C-sis PS-AODN and C-sis AODN degradation rate were 23.7% and 59.6% in an hour and day 6, and 43.3% and 53.1% in an hour and a day, respectively. Conclusion C-sis PS - AODN has good resistance to nuclease in serum, and is able to be used clinically. |
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| Keywords: | C-sis proto-oncogene Antisense oligodeoxynucleotides Antisense phosphorthuoate oligodeoxynu-cleotides Stability |
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