中耳胆脂瘤中XIAP与Caspase-3的相关性研究

目的研究凋亡抑制蛋白XIAP、Caspase-3在胆脂瘤中的表达及胆脂瘤中的凋亡情况,探讨三者之间的关系,揭示XIAP、Caspase-3、凋亡在胆脂瘤发生发展中的作用,深入认识胆脂瘤发病机制,并为胆脂瘤的临床治疗方法提供新的思路。方法①运用免疫组织化学两步法检测25例胆脂瘤标本及10例外耳道正常皮肤组织中XIAP及Caspase-3的表达。②采用末端转移酶介导的原位缺口末端标记染色(TUNEL)技术进行检测两者组织的凋亡状态。结果①与正常上皮比较,胆脂瘤上皮中的XIAP蛋白表达明显下调(Z=2.411,P<0.05)。②胆脂瘤上皮中,Caspase-3的表达及细胞凋亡状况显著高于正常上皮(Zc=-2.877,Zt=-2.712,P<0.01)。③在胆脂瘤上皮中,XIAP表达与Caspase-3及凋亡均呈负相关(P<0.01),Caspase-3与凋亡呈正相关(P<0.01)。结论XIAP、Caspase-3的表达可能在胆脂瘤的发生、发展过程中起重要作用,它们参与胆脂瘤上皮的凋亡调控过程,可能是胆脂瘤的发病机制中一个重要因素。

The expression of XIAP in middle ear Cholesteatoma and the relationship with Caspase-3

Objective The purpose of this study is to examine the expression of XIAP,Caspase-3 and the apoptosis states in middle ear cholesteatoma, trying to discuss the relationship of XIAP, Caspase-3 and apoptosis states in middle ear cholesteatoma, investigate the possible role of XIAP and Caspase-3 in middle ear cholesteatoma and further explore the pathogenesis of middle ear cholesteatoma. Methods ①Specimens of 25 cholesteatoma and 10 normal skin collected from the externalear auditory canal were examined by immunohistochemical method using polyclonal antibodies of XIAP and Caspase-3. ②The apoptosis states of the 2 tissues were determined by TUNEL method. Results ③ Comparing with normal externalear auditory canal skin,the expression of XIAP protein decreased obviously in cholesteatoma （Z=-2.411,P〈0.05）.②The expression of Caspase-3 and apoptosis in cholesteatoma tissue were significant higher than that in normal externalear auditory canal skin（Zc=-2.877,Zt=-2.712,P〈0.01）.③In cholesteatoma epithelium ,the expression of XIAP was inversely correlated with Caspase-3 and apoptosis （P〈0.01）. There was a direct correlation between Caspase-3 and apoptosis （P〈0.01）. Conclusion The expressions of XIAP and Caspase-3 in the cholesteatoma epithelium suggest that stheir important roles for in the formation and development of cholesteatoma. They participate the regulatory procedure of apoptosis and may be a considerable factor of nosogenesis in cholesteatoma.