竹胎粉辅助卟啉铁改善营养性贫血的实验观察

目的:对竹胎粉的功效成分进行检测,观察卟啉铁复合竹胎粉辅助改善营养性贫血的效果,并对其安全性进行评价。方法:采用放射免疫法测定竹胎粉中造血相关因子结构类似物的含量。用电感偶合等离子体质谱(ICTMS)检测微量元素的量。同时建立营养性贫血大鼠动物模型,灌胃竹胎粉加卟啉铁,观察血红蛋白变化。急性毒性试验采用小鼠最大灌胃量,观察有无毒性反应。结果:竹胎粉中含造血因子红细胞生成素(EPO)、IL-1、IL-2结构类似物,刺激或参与造血功能的微量元素Mn、Fe、Zn、Cu等,以及多种氨基酸成分。动物试验表明,给予卟啉铁3.17mg/kgbw复合竹胎粉11.5g/kgbw组的大鼠血红蛋白、红细胞压积、体重与对照组相比均有显著性差异(P<0.05),单纯使用卟啉铁组有效剂量为6.34mg/kgbw。急性毒性试验未见不良反应。结论:竹胎粉能够辅助卟啉铁改善营养性贫血。

THE EXPERIMENTAL OBSERVATION ON THE EFFECT OF PORPHYRIN IRON COMBINED WITH BAMBOO EMBRYO POWDER IN NUTRITIONAL ANEMIA THERAPY

Objective: To observe the effect of bamboo embryo powder aiding porphyrin iron in treatment of nutritional anemia. At the same time its acute toxicity was tested. Method:The factors related to hematopoiesis, for example EPO、IL-1 、IL-2 were detected by radioimmunoassay and trace elements by ICTMS. Bamboo embryo powder combined with porphyrin iron was applied for therapy of nutritional anemia in rats. The acute toxicity assay was used. Results:There were EPO、IL-1 、IL-2 and Fe、Cu、Zn、 Mn in bamboo embryo powder. Bamboo embryo powder combined with porphyrin iron was better than the single porphyrin iron of same dose for treatment of nutritional anemia in rats . The effective dose of single porphyrin iron was 6.34 mg/kg bw, but only 3.17 mg/kg bw was needed if used in combination with 11.5 g/kg bw bamboo embryo power. Acute toxicity test showed no adverse reaction. Conclusion: Bamboo embryo powder could aid porphyrin iron in nutritional anemia therapy.