兔胆囊结石成石过程中脂代谢的变化及高密度脂蛋白制剂对其影响的研究

为研究高胆固醇膳食诱发兔胆囊胆固醇结石模型的脂代谢变化，以及高密度脂蛋白（ＨＤＬ）对其脂代谢及成石的影响，将动物随机分为对照组、高胆固醇膳食组及高胆固醇膳食＋ＨＤＬ注射组，观察各组血浆脂质、胆汁中胆固醇、血卵磷脂胆固醇酰基转移酶（ＬＣＡＴ）活性及血和胆汁中胆汁酸及成石率的变化。结果显示：动物体内胆固醇等脂质的消除障碍导致体内脂质的大量堆积以及清除紊乱，是高胆固醇膳食诱发兔胆囊结石脂代谢变化的主要特点，胆汁中胆固醇与胆汁酸的清除比例失调可能导致成石性胆汁形成；尽管外源性ＨＤＬ制剂能够升高血浆ＨＤＬ２－Ｃ／ＨＤＬ３－Ｃ的比值，增加ＬＣＡＴ的活性从而促使ＨＤＬ成熟，并能降低肝脏的胆固醇含量，但未能降低成石率。结果表明外源性ＨＤＬ制剂对兔胆囊结石形成中脂代谢有一定的影响，但抗成石作用不明显

Changes of Lipids Metabolism During Gallstone Formation in Rabbit Model and the Effects on these Changes by High Density Lipoprotein Preparation Injection

To study the changes of lipids metabolism during gallstone formation and the effects of the exogenous high density lipoprotein (HDL) preparation on the formation of gallbladder stone in rabbit model in which the stones were induced by high cholesterol (HC) diet. The plasma lipids, the bile cholesterol, the activity of palsma lecithine cholesterol acyltransferase (LCAT), the bile acids in both bile and plasma, and the incidence of stone formation were investigated in the control group, HC group, and HC HDL group (in this group the rabbits were given HDL preparation intravenously regularly).The results showed that the lipids metabolic disorder was characterized by a large amount of cholesterol accumulated in the the rabbit's body and when the liver cleaned the accumulated cholesterol, the ratio of biliary cholesterol to bile acids was significantly increased. The latter could cause the production of lithogenic bile. Thought HDL preparation could decrease hepatic cholesterol and increase the level of plasma HDL 2 C and the activity of LCAT, it could not lower the incidence of gallstone formation. The results suggest that the HDL preparation had some effects on lipids metabolism, but no significant effect on gallstone formation in rabbit model has been evidenced, so the problem awaits further studies.