| CVB3cDNA重组质粒pGP51B转化作用的实验研究 |
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| 引用本文： | 曲章义 牛美娟. CVB3cDNA重组质粒pGP51B转化作用的实验研究[J]. 哈尔滨医科大学学报, 1996, 30(5): 408-410 |
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| 作者姓名： | 曲章义 牛美娟 |
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| 作者单位： | 微生物学教研室 |
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| 摘 要： | 以大肠杆菌ＨＢ１０１菌株为受体菌，研究了ＣａＣｌ２浓度，转化时间及转化 温育时间对柯萨奇Ｂ组病毒３型ｃＤＮＡ重组质粒ｐＧＰ５１Ｂ转化作用的影响。实验结果表明，ＣａＣｌ２浓度和转化时间对转化率的影响极为显著，而转化后的能时间对转化率的影响不明显。
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| 关 键 词： | 重组质粒 转化 柯萨奇病毒 CVB3 cDNA |
Study on the Transformation of Recombinant Plasmid pGP51B Deveried from Coxsackie Virus cDNA |
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| Qu Zhangyi,et al. Study on the Transformation of Recombinant Plasmid pGP51B Deveried from Coxsackie Virus cDNA[J]. Journal of Harbin Medical University, 1996, 30(5): 408-410 |
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| Authors: | Qu Zhangyi et al |
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| Affiliation: | Department of Microbiology |
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| Abstract: | The effects of transformation conditions,which included CaCl2 concentration,transformation time and incubation time,on the transformation of recombinant plasmid pGP51B were investigated in this paper.pGP51B was a recombinant molecule of Coxsackie virus B3 cDNA fragment with the vector plasmid pGEM-1.The result showed that the effects of different CaCl2 concentration and transformation time on the transforn1ation frequency were significant differences,but the effect of incubation time after transformation was insignificant.The best condition for pGP51B transformation in this experiment was to prepare the competent host cells with 75mmol/L CaCl2 then transformating in ice bath for 30min following the direct selection on Amp+LB plate. |
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| Keywords: | Recombinant plasmid Transformation Coxsackie virus |
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