ERCC1基因多态性与肝癌易感性研究

目的 研究ERCCl基因多态性与福州地区人群肝癌易感性之间关系,及其与环境因素交互作用对肝癌发生的影响.方法 采用病例对照研究收集病例和对照暴露信息,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术对ERCCl-C8092A基因多态性进行检测;通过广义线性模型模拟ERCC1-C8092A基因多态性与肝癌主要环境危险因素交互作用的结构模型.结果 以ERCC1-C8092ACC作为参照,ERCC1-C8092ACA/AA基因型可以增加原发性肝癌的发病危险性(OR=3.789,95%CI:2.792～5.142);ERCCl-C8092A突变与饮沟塘水、乙型肝炎病毒感染的联合作用最适模型均为超相加模型,与霉变食品联合作用为超相乘模型.结论 ERCC1-C8092A多态可能与肝癌遗传易感有关,并与肝癌主要环境因素间存在协同作用.

Relationship between genetic polymorphism of ERCC1 and susceptibility to liver cancer

Objective To investigate the association between genetic polymorphisms of ERCCl-C8092A and susceptibility to liver cancer, as well as the gene-environmental interaction on the etiology of liver cancer in Fuzhou. Methods A case-control study was conducted to collect the information on environmental exposure while genetic polymorphism of ERCC1 was analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. General relative risk regression models were further applied to fit the interaction between genetic polymorphisms of ERCC1-C8092A and the environmental factors of liver cancer. Results ERCC1-C8092A variant genotypes were associated with significant increasing risk of liver cancer adjusted odds ratio (OR= 3.789,95%CI:2.792-5.142 ), compared to the wild-type homozygote. Data from the analysis of interaction showed that genetic polymorphism of ERCC1-8092A appeared superadditive interaction with drinking pond-ditch or with hepatitis B, and super-multiplicative interaction with eating moldy food. Conclusion The point mutation in ERCC1-8092A was possibly susceptible to liver cancer, and related synergistically with other risk factors in hepatocelluar carcinogenesis in Fuzhou.