| HPLC-MS/MS法测定新生儿血浆中奥美拉唑的浓度 |
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| 引用本文： | 郑丽亚,刘向楠,张智勇,徐敬朴,段立广,郑旭光,张志清,周春华. HPLC-MS/MS法测定新生儿血浆中奥美拉唑的浓度[J]. 中国临床药理学杂志, 2018, 0(23): 2761-2764 |
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| 作者姓名： | 郑丽亚 刘向楠 张智勇 徐敬朴 段立广 郑旭光 张志清 周春华 |
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| 作者单位： | 河北医科大学第二医院药学部;河北省胸科医院药械处;河北医科大学第一医院药学部 |
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| 摘 要： | 目的建立一种测定新生儿血浆中奥美拉唑浓度的HPLC-MS/MS法。方法新生儿血浆样品用乙腈沉降蛋白后,以兰索拉唑为内标,色谱柱为C18(4. 6 mm×150. 0 mm,3. 5μm),流动相为乙腈-水(0. 1%甲酸)=85∶15,流速为0. 3 m L·min-1,柱温为35℃,进样量为10μL。用电喷雾离子化源,正离子方式,多反应监测(MRM)扫描方式进行监测。考察该方法的专属性、标准曲线与定量下限、精密度与回收率,以及稳定性。结果血浆中奥美拉唑的浓度在5～4000 ng·m L-1内线性关系良好,标准曲线为y=1. 20×10-3x+2. 06×10-2(r=0. 998 7),定量下限为5 ng·m L-1。批内、批间RSD均小于15%,相对偏差为-7. 36%～3. 62%,提取回收率为77. 94%～93. 12%,无明显的基质效应。结论该方法简便快速,灵敏度好,准确度高,专属性强,适用于新生儿血浆中奥美拉唑浓度的测定。
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| 关 键 词： | 奥美拉唑 新生儿 HPLC-MS/MS 血药浓度 |
Determination of omeprazole concentration in neonates plasma by HPLC-MS/MS |
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| ZHENG Li-ya,LIU Xiang-nan,ZHANG Zhi-yong,XU Jing-pu,DUAN Li-guang,ZHENG Xu-guang,ZHANG Zhi-qing,ZHOU Chun-hua. Determination of omeprazole concentration in neonates plasma by HPLC-MS/MS[J]. The Chinese Journal of Clinical Pharmacology, 2018, 0(23): 2761-2764 |
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| Authors: | ZHENG Li-ya LIU Xiang-nan ZHANG Zhi-yong XU Jing-pu DUAN Li-guang ZHENG Xu-guang ZHANG Zhi-qing ZHOU Chun-hua |
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| Affiliation: | (Department of Pharmacy, The Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Department of Pharmacy and Instrument, Hebei Chest Hospital, Shijiazhuang 050000, China;Department of Pharmacy, The First Hospital of Hebei Medical University, Shijiazhuang 050000,China) |
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| Abstract: | ZHENG Li-ya;LIU Xiang-nan;ZHANG Zhi-yong;XU Jing-pu;DUAN Li-guang;ZHENG Xu-guang;ZHANG Zhi-qing;ZHOU Chun-hua(Department of Pharmacy, The Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Department of Pharmacy and Instrument, Hebei Chest Hospital, Shijiazhuang 050000, China;Department of Pharmacy, The First Hospital of Hebei Medical University, Shijiazhuang 050000,China) |
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| Keywords: | omeprazole neonate HPLC-MS /MS plasma concentrationof drug |
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