| 大鼠离体肝脏5′-NT和ACP活性与PMI的相关性研究 |
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| 引用本文： | 闫宜锋,李永宏.大鼠离体肝脏5′-NT和ACP活性与PMI的相关性研究[J].皖南医学院学报,2008,27(3):170-171. |
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| 作者姓名： | 闫宜锋 李永宏 |
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| 作者单位： | 皖南医学院,法医学系,安徽,芜湖,241002 |
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| 摘 要： | 目的:探讨大鼠死后肝脏内5′-核苷酸酶(5-′Nu-cleotidase,下称5-′NT)和酸性磷酸酶(Acid phosphatase,下称ACP)活性变化与死后经过时间(Postmortem interval,PMI)的相关性。方法:应用分光光度法检测18℃和28℃下大鼠死后肝脏5-′NT和ACP的活性。结果:大鼠死后0~21 h,其肝脏内5-′NT由150 IU/L逐渐降至0,且与时间呈明显的负相关,回归方程为:PMI=23.18-0.774X5-′NT(r=-0.882,P<0.01);而ACP与时间无明显相关性。结论:5′-NT的降解规律可作为推断PMI的参考指标。
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| 关 键 词： | 法医病理学 死后经过时间 肝脏 5′-核苷酸酶 酸性磷酸酶 |
| 文章编号： | 1002-0217(2008)03-0170-02 |
| 修稿时间： | 2007年4月18日 |
Experimental study on the correlation between postmortem intervals and 5′-NT or ACP in rats′ liver in vitro |
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| YAN Yi-feng,LI Yong-hong.Experimental study on the correlation between postmortem intervals and 5′-NT or ACP in rats′ liver in vitro[J].Acta Academiae Medicinae Wannan,2008,27(3):170-171. |
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| Authors: | YAN Yi-feng LI Yong-hong |
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| Abstract: | Objective:To investigate the correlation between postmortem interval(PMI) and 5′-Nucleotidase(5′-NT) or acid phosphate(ACP).Methods: 5′-NT and ACP activities in the rats′ liver in vitro were measured at the temperature of 18 ℃ and 28 ℃ by using spectrophotometry. Results: 5′-NT activity decreased gradually with the extension of postmortem intervals from 0 to 21 hours after death. Statistical analysis showed that the regression equation was negative to the interval(PMI =23.18-0.774X5′-NT;R=0.872)(P<0.01) but had no correlation between ACP and PMI.Conclusion:Decrease in 5′-NT activity may be used as the relatively objective parameter for PMI estimation. |
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| Keywords: | forensic pathology postmortem interval(PMI) liver 5′-Nucleotidase(5′-NT) acid phosphatidase(ACP) |
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