补体C1q及抗C1q抗体与系统性红斑狼疮及狼疮肾炎相关性的研究

目的 检测系统性红斑狼疮（SLE）患者血清补体C1q及抗C1q抗体（C1qAb）水平，比较血清C1q与C1qAb之间的相关性；分析血清C1q及C1qAb水平与SLE、狼疮肾炎（LN）以及SLE病情活动的相关性。方法 采用单向免疫扩散法（SRID）检测血清C1q，酶联免疫吸附法（ELISA）检测血清C1qAb。结果 SLE患者的血清C1q水平显著低于其他对照组，而血清C1qAb水平显著高于其他对照组。其中LN患者的血清C1q水平显著低于非LN的SLE患者、血清C1qAb水平显著高于非LN的SLE患者。SLE患者血清C1qAb与C1q之间呈显著负相关。病情活动期的SLE患者血清C1q水平显著低于稳定期患者；血清C1qAb水平显著高于稳定期患者。结论 血清C1q的降低及血清C1qAb的升高与SLE相关。血清C1qAb的升高，可能是导致血清C1q降低的主要原因之一。血清C1q、C1qAb水平与SLE患者肾脏损害显著相关。血清C1q、C1qAb水平与病情活动显著相关。

Study of the complement 1q and its autoantibodies and their relationships with systemic lupus erythematosus and lupus nephritis

Objective To detect the serum level of complement 1q (C1q) and anti-C1q autoantibodies (C1qAb) in systemic lupus erythematosus (SLE) patients to analyze the correlation of serum level of C1q and C1qAb with renal lesion and disease activity in SLE. Methods The serum level of C1q and C1qAb were detected by single radial lmnmnodiffusion and enzyme-linked immunosorbent assay respectively. Results The serum level of C1q in SLE patients was significantly lower than that of the control groups. The serum level of C1qAb in SLE patients was significantly higher than that of the control groups. There was a strong correlation between the serum level of C1q and C1qAb in SLE patients. SLE patients in flare stage showed a significantly higher level of serum C1qAb and a lower level of serum C1q than stable patients. Lupus nephritis(LN) patients showed a significantly higher level of serum C1qAb and a lower level of serum C1q than non-LN patients. Conclusions Low level of serum C1q and the high level of serum C1qAb are correlated with SLE. The serum level of C1q and C1qAb are significantly correlated with renal lesion and disease activity of SLE patients.