聚合酶链反应与常规方法检测牙龈卟啉单胞菌的比较研究

目的 使用聚合产链反应检测龈下菌斑的牙龈卟啉菌,并与常规的培养法和间接免疫荧光法相比较,方法 设计纤毛亚单位蛋白基因内的一对引物,用ＰＣＲ对９２个龈证菌斑标本进行扩增,与其他两种方法的检出率进行比较。结果 相同的菌斑标本,ＰＣＲ的检出率为９４．６％,培养法为４８．９％,间接免疫荧光法为６８．５％,前者明显高于后两者。结论对牙周病致病菌的检测,ＰＣＲ较培养法和间接免疫荧光法具有较高的敏感性。本项研究

A comparison of polymerase chain reaction with other two widely used methods in detection of Porphyromonas gingivalis

Objective To compare the efficiency of polymerase chain reaction (PCR) with cultural method and indirect immunofluorescence(IF) in detection of Porphyromonas gingivalis (P g) in oral plaque samples Methods 92 subgingival plaque samples were collected from patients of three kinds of periodontal disease A program using PCR to detect Fimbrilin gene (Fim A) was designed to detect P g The efficiency of this PCR method was compared with cultural method and IF Results The positive rate of PCR was 94 6%, while that of cultural method and IF was only 48 9% and 68 5% respectively The difference was significant in statistical analysis ( P <0 001) Conclusion PCR is more sensitive than culture method and IF in detecting pathogens of periodontal diseases The study provides a simplified and efficient method for oral clinical use