Assessment of Multimeric Structure and Ristocetin-Induced Binding to Platelets of Von Willebrand Factor Present in Cryoprecipitate and Different Factor VIII Concentrates |
| |
Authors: | MF López-Fernández MD C López-Berges M Corral JR García-Talavera A López Borrasca and J Batlle |
| |
Institution: | Division of Hematology-Hemotherapy, Hospital Clínico, University of Salamanca, Spain;Department of Nuclear Medicine, Hospital Clínico, University of Salamanca, Spain |
| |
Abstract: | The multimeric structure of von Willebrand factor (vWF) and its ristocetin-induced binding to platelets, using a simple and very sensitive radiomonoclonal antibody-labeled vWF method, was compared in normal plasma, single-donor cryoprecipitate (CP) and five different antihemophilic factor (AHF) concentrates. All the AHF showed a lack of larger vWF multimers, an abnormal 'triplet' pattern, and much lower vWF binding to platelets than that of plasma or CP, vWF being the lowest for those with a lesser proportion of larger vWF multimers. These results suggest that the combination of vWF multimeric analysis and the radiomonoclonal-labeled vWF method may be very useful in the assessment of AHF preparations. |
| |
Keywords: | |
|
|