去甲肾上腺素/烧伤血清诱导大鼠神经胶质细胞VEGF基因表达

目的： 观察血管内皮细胞生长因子（VEGF）在去甲肾上腺素（NE）/烧伤血清诱导神经胶质细胞时基因表达变化。 方法： 用免疫荧光法观察NE/烧伤血清刺激神经胶质细胞后24 h VEGF在细胞内的分布。用免疫蛋白印迹法检测细胞VEGF蛋白表达。用荧光定量聚合酶联反应（PCR）法检测细胞VEGF mRNA表达。 结果： ①高剂量NE（50 μmol/L）刺激时，神经胶质细胞胞浆内绿色荧光增强，烧伤血清刺激后绿色荧光明显增强，当烧伤血清加高剂量NE刺激时绿色荧光显著增强。②烧伤血清组细胞VEGF蛋白表达增加，烧伤血清加中剂量（20 μmol/L）、高剂量NE组VEGF的蛋白表达明显增加。③烧伤血清刺激后，细胞VEGF mRNA表达增加，烧伤血清与NE同时刺激时，随着NE剂量的增加，细胞中VEGF mRNA表达明显增加。 结论： NE/烧伤血清能够诱导神经胶质细胞VEGF的基因表达增强，提示烧伤应激因素诱导神经胶质细胞分泌VEGF可能是烧伤后脑水肿形成的一个重要因素。

VEGF gene expression in norepinephrine/ burn serum-induced rat astrocytes

AIM: To investigate the changes of gene expression of vascular endothelial growth factor (VEGF) in norepinephrine/ burn serum-induced astrocytes. METHODS: Immunofluorescence staining was used to show the distribution of VEGF in astrocytes after 24 h using norepinephrine/burn serum stimulation. Western blotting was used to detect protein expression of VEGF. Real time PCR was used to investigate expression of VEGF mRNA. RESULTS: ① Green fluorescence of protein expression of VEGF in astrocytes was increased when treated with high dose norepinephrine (50 μmol/L). Green fluorescence of protein expression of VEGF in astrocytes was increased distinctness after burn serum stimulation. Green fluorescence protein expression of VEGF in astrocytes was increased significantly when high dose norepinephrine combined with burn serum stimulation was added. ② VEGF protein expression in burn serum stimulating group was increased, and VEGF protein expression was significantly increased when burn serum was added during moderate (20 μmol/L), high dose norepinephrine stimulation. ③ Expression of VEGF mRNA was increased in burn serum-treated astrocytes. Expression of VEGF mRNA was increased significantly when norepinephrine-stimulated astrocytes exposed to barn serum, and as norepinephrine dose increases gradually. CONCLUSION: Norepinephrine and burn serum play an important role in inducing VEGF protein expression in astrocytes, suggesting that stress reaction of postburn is an important cause in inducing brain edema by excreting VEGF in astrocytes.