2012年云南省及缅甸籍入境健康儿童肠道病毒的分子生物学检测

目的了解2012年云南省和缅甸籍入境健康儿童肠道病毒（Enterovirus，EV）带毒情况及病毒型别。方法采集6个边境设区的市（州、地区，下同）10个县和7个非边境市10个县〈15岁健康儿童粪便标本600份（单份粪便标本，其中缅甸籍入境儿童100份），进行病毒分离和基因测序定型。结果600份粪便标本共检测到EV53株，带毒率为8．83％。其中脊髓灰质炎病毒5株，阳性率0．83％，均为疫苗株，未发现脊灰野病毒。检测到非脊灰肠道病毒（Non．polioEV，NPEV）48株，阳性率8．00％，其中人类肠道病毒（HumanEV，HEV）A组5株（3个血清型，占10．42％），HEVB组（GroupB）（HEV．B）42株（14个血清型，占87．50％），HEVC组6株（4个血清型，占11．32％），未分离到HEVD组。结论2012年600份健康儿童粪便标本中，从中国籍儿童分离到EV48株，分离率8．00％，缅甸籍儿童中分离到EV5株，分离率0．83％，中国籍儿童EV携带率较高，并以HEV．B组为主。

Molecular Test of Enteroviruses for Healthy Children in Yunnan Province and the Border Areas with Myanmar, 2012

Objective To explore the enteroviruses （EV） surveillance among healthy children under 15 years old in Yunnan province and the border areas with Myanmar in 2012. Method The stool samples were collected from the healthy children from 10 counties from non-border areas and 10 counties in the border areas with a total of 600 samples, virus isolation were conducted for the all 600 samples and positive samples were sequenced. Result 5 strains of po]ioviruses were detected with a 0.83% isolation rate, which are belong to vaccine strain and 48 non-polio EV （NPEVs） were detected with an isolation rate of 8.00%. Of which, 5 strains belong to Human EV Group A （HEV-A, 3 serotypes, accounting for 10.42% ）, 42 strains belong to HEV-B 14 serotypes （accounting for 87.50% ）, 6 strains belong to HEV-C 4 serotypes （accounting for 11.32% ）, HEV-D viruses were not isolated. Conclution Our results showed that 48 strains of EVs were isolated from Chinese children with an isolation rate of 8.00%, 5 strains of EVs were isolated from Myanmar children with an isolation rate of 0.83 %. In China, the EV isolation rate is higher than the Myanmar children, with HEV-B viruses being the predominant viruses.