洛伐他汀促进颌面部骨折愈合过程中BMP-2表达的研究

目的探讨洛伐他汀促进颌骨骨折愈合过程中对骨形成蛋白-2（BMP-2）的影响。方法健康家兔24只,于颌骨制造骨折模型,再随机分为给药组和对照组,每组12只,给药组手术次日给予10mg/（kg·d）洛伐他汀灌胃,疗程为28天,对照组给予安慰剂。利用RT-PCR及Western blot检测骨折愈合过程中给药组和对照组骨折后3、7、10、14、21和28天骨痂中BMP-2的表达,同时应用ELISA检测血清中BMP-2的含量。结果 RT-PCR结果显示,给药组BMP-2基因表达高峰出现在第7天,对照组出现在第14天,且峰值较低。同时Western blot结果显示,术后7~14天骨痂中BMP-2蛋白给药组明显高于对照组,术后21~28天给药组与对照组没有明显差异。血清学检测两组BMP-2升高,在术后14天含量达到高峰,且给药组明显高于对照组（P〈0.05）。结论洛伐他汀明显促进颌骨骨折愈合过程中BMP-2的合成与分泌,进而有利于骨折的愈合。

Effect of Lovastatin on BMP-2 During Fracture Healing Process of Mandibular Fracture

Objective To explore the effect of Lovastatin on BMP-2 during fracture healing process of mandibular fracture. Methods The fracture models of mandibular were established in with 24 rabbits. The rabbits were ran- domly and equally divided into medication group and control group, in which the rabbits were lavaged with Lovas- tatin of 10 mg/kg per day and placebo for 4 weeks since the next day after fracture. The expression of BMP-2 pro- tein in bony callins were determined by PT- PCR and Western blot method on days 3,7,10,14,21, and 28 of fracture. The concentration of BMP-2 in serum was analyzed by ELISA. Results On days of 7 of fracture, BMP-2 gene expression was reach at the peak in medication group. However, the gene expression of BMP-2 in control group was delayed （on days of 14） in comparison with medication group. The results of HE stain showed that the positive cells of BMP-2 in experiment groups were higher than that in control group on days 7 and 14 （P 〈0.05）, but on day 28 there was no different compared to control group （P 〉0.05）. BMP-2 in serum were detected after fracture, and high expression of BMP-2 was detected on day 14 after fracture in medication group, which was higher than that in control group （ P 〈 0.05 ）. Conclusion Lovastatin could promote the synthesis and secretion of BMP-2 during fracture healing, which can accelerate healing process of mandubular fracture.