傣药山楂内金胶囊中连翘苷含量测定

目的：研究建立高效液相色谱测定傣药山楂内金胶囊中连翘苷含量的方法。方法：HPLC法，采用C18色谱柱；以乙腈为流动相A，水为流动相B，按规定条件梯度洗脱；检测波长为230nm。供试品溶液的制备方法为：甲醇超声提取，大孔树脂和中性氧化铝柱（内径2cm，用水湿法装柱；下部为100～200目中性氧化铝5g；上部为高度6cm的预处理好的D101大孔树脂）纯化。结果：精密度试验RSD为2．17％（n=6）；重复性试验RSD为1．76％（n=6）；准确度（印收率）试验平均回收率为96．8％，RSD为3．13（n=6）；阴性无干扰。结论：本方法操作简便，有效排除杂质干扰，降低基线，可作为山栏内金胶囊的质量控制方法。

Content Determination of Forsythin in Shanzha Neijin Capsules by HpLc

Objective: To establish a HPLC method for content determination of forsythin in Shanzha Neijin capsules.Method: The HPLC was carried out on C18 column.The mobile phase consisted of acetonitrile(A) and water(B).Gradient program was adopted.The detective wavelength was 230nm.The tested solution was prepared as follows:extracted with MeOH by supersonic,purified by maroporous adsorption resin neutral alumina column chromatography(diameter is 2 cm,Bottom is neutral alumina 5g;Top is D101 maroporous adsorption resin 6 cm in altitude).Results:Precision test RSD=2.17%(n=6);Repeatability test RSD=1.76%(n=6);The average recovery of Accuracy(recovery rate) test is 96.8%,RSD=3.13%(n=6);There were no interference in the negative test.Conclusion: The present method is accurate,reliable,simple,convenient and with good reproducibility,the sample was purified completely,and little interference and it can be used for quality control of Shanzha Neijin Capsules.