联合检测HBV前S1抗原和核心抗原的临床意义 |
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引用本文: | 章述军,田德英,李晖,周健,袁权,葛胜祥,张军,夏宁邵. 联合检测HBV前S1抗原和核心抗原的临床意义[J]. 中西医结合肝病杂志, 2008, 18(2): 74-76 |
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作者姓名: | 章述军 田德英 李晖 周健 袁权 葛胜祥 张军 夏宁邵 |
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作者单位: | 1. 华中科技大学同济医学院附属同济医院感染科,湖北,武汉,430030 2. 厦门大学国家传染病诊断试剂与疫苗工程技术研究中心 |
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基金项目: | 国家高技术研究发展计划(863计划) |
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摘 要: | 探讨联合检测血清HBV前s1抗原(preSl)和核心抗原(HBcAg)(均为HBV核酸相关抗原,nucleic acids related antigen,HBV NRAg)的意义及临床价值。方法:采用ELISA法对393份HBsAg、HBV DNA双阳性的血清和612份HBsAg阴性血清进行HBV NRAg检测,所有标本均采用多区段巢式PCR确认阳性、阴性,采用荧光定量PCR法进行HBV DNA定量分析。结果:393份HBsAg、HBV DNA双阳性血清中,HBV NRAg阳性为382份,其阳性率为97.2%;612份HBsAg阴性的血清标本中,609份确认为HBV DNA阴性,其中检出2份HBV NRAg阳性,607份为阴性,其HBV NRAg的阴性率为99.7%(607/609),另3份HBsAg阴性血清HBV DNA阳性者,其HBV NRAg均为阳性。结论:联合检测preSl和HBcAg的HBV NRAg可作为临床HBV感染的筛选及判断HBV复制的有意义的补充项目。
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关 键 词: | 肝炎病毒 乙型 前S1抗原 核心抗原 |
修稿时间: | 2007-11-14 |
Clinical significance of combined detecting hepatitis B virus preS1 antigen and core antigen |
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Affiliation: | ZHANG Shu-jun, TIAN De-ying, LI Hui, et al. 1. Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology ( Wuhan Hubei, 430030 ) China |
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Abstract: | Objective: To study the clinical significance of combined detecting preS1 antigen and hepatitis B core antigen (HBV NRAg, nucleic acids related antigen) . Methods: Serum sample were collected from 393 patients positive for HBsAg and HBV DNA, and 612 samples negative for HBsAg. HBV pre-S1 combined with HBcAg were detected by HBV NRAg ELISA HBV DNA were detected by fluorescent quantitation polymerase chain reaction (FQ-PCR) and confirmed by nPCR. Results: Of 393 samples positive for HBsAg and HBV DNA, 382 (97.2%) were positive for HBV NRA. In 612 samples negative for HBsAg, 609 samples were negative for HBV DNA. The specificity of HBV NRAg was 99. 7% (607/609) . In addition, the other 3 serum samples with negative for HBsAg and positive for HBV DNA their HBV NRAg were positive. Conclusion: HBV NRAg ELISA can be a useful supplementary for clinical judgement of HBV infection and replication. |
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Keywords: | hepatitis B virus preS1 antigen hepatitis B core antigen |
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