联合检测HBV前S1抗原和核心抗原的临床意义

探讨联合检测血清HBV前s1抗原（preSl）和核心抗原（HBcAg）（均为HBV核酸相关抗原，nucleic acids related antigen，HBV NRAg）的意义及临床价值。方法：采用ELISA法对393份HBsAg、HBV DNA双阳性的血清和612份HBsAg阴性血清进行HBV NRAg检测，所有标本均采用多区段巢式PCR确认阳性、阴性，采用荧光定量PCR法进行HBV DNA定量分析。结果：393份HBsAg、HBV DNA双阳性血清中，HBV NRAg阳性为382份，其阳性率为97．2％；612份HBsAg阴性的血清标本中，609份确认为HBV DNA阴性，其中检出2份HBV NRAg阳性，607份为阴性，其HBV NRAg的阴性率为99．7％（607／609），另3份HBsAg阴性血清HBV DNA阳性者，其HBV NRAg均为阳性。结论：联合检测preSl和HBcAg的HBV NRAg可作为临床HBV感染的筛选及判断HBV复制的有意义的补充项目。

Clinical significance of combined detecting hepatitis B virus preS1 antigen and core antigen

Objective： To study the clinical significance of combined detecting preS1 antigen and hepatitis B core antigen （HBV NRAg, nucleic acids related antigen） . Methods： Serum sample were collected from 393 patients positive for HBsAg and HBV DNA, and 612 samples negative for HBsAg. HBV pre-S1 combined with HBcAg were detected by HBV NRAg ELISA HBV DNA were detected by fluorescent quantitation polymerase chain reaction （FQ-PCR） and confirmed by nPCR. Results： Of 393 samples positive for HBsAg and HBV DNA, 382 （97.2%） were positive for HBV NRA. In 612 samples negative for HBsAg, 609 samples were negative for HBV DNA. The specificity of HBV NRAg was 99. 7% （607/609） . In addition, the other 3 serum samples with negative for HBsAg and positive for HBV DNA their HBV NRAg were positive. Conclusion： HBV NRAg ELISA can be a useful supplementary for clinical judgement of HBV infection and replication.