Inhibition of inflammatory angiogenesis in rats by loco-regional administration of hydrocortisone and protamine |
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Authors: | D. Foschi L. Castoldi F. Corsi E. Radaelli E. Trabucchi |
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Affiliation: | (1) First Department of Surgery and Wound Healing Center, University of Milan, Ospedale Maggiore Policlinico IRCCS, via F. Sforza 35, I-20157 Milano, Italy |
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Abstract: | We have studied the antiangiogenetic effects of hydrocortisone and protamine given intra-arterially. The cornea of male, Sprague-Dawley rats were cauterized with silver nitrate. The following treatments were given :30 g hydrocortisone topical (t.p.), b.i.d., 50 mg/kg/day intraperitoneally (i.p.) or intra-arterially (i.a.), 10 mg/ kg/day protamine i.p. or i.a. Saline was administered to the control groups. In separate experiments we also evaluated the anti-inflammatory effects of hydrocortisone, i.p., on the cauterized corneas.Five days after cauterization, the animals were killed, exsanguinated and India ink was injected to show the network of neovessels. The percentage area of the cornea covered by neovessels was measured morphometrically and evaluated statistically. Hydrocortisone t.p. (–84%), i.a. (–60%) and protamine i.a. (–44%) significantly inhibited angiogenesis in the cauterized cornea. Either drugs, i.p., had any antiangiogenetic effects, but hydrocortisone significantly reduced cell infiltration of the corneas. The results suggest that locoregional administration of antiangiogenetic drugs might be clinically useful. |
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Keywords: | Corticosteroids Protamine Angiogenesis Inflammation Vessels |
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