| 精浆/精子乳酸脱氢酶活性与男性不育的相关性 |
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| 引用本文： | 刘雅峰,欧建平,周灿权,徐艳文,方丛,王琼,钟依平,苗本郁. 精浆/精子乳酸脱氢酶活性与男性不育的相关性[J]. 中国优生与遗传杂志, 2010, 0(2): 121-122 |
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| 作者姓名： | 刘雅峰 欧建平 周灿权 徐艳文 方丛 王琼 钟依平 苗本郁 |
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| 作者单位： | 中山大学附属第一医院生殖中心; |
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| 基金项目： | 广东省科技计划项目,编号:2007B030500001; 广东省科技计划项目,编号:2007B030502007 |
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| 摘 要： | 目的探讨精浆及精子中乳酸脱氢酶同工酶X(LDH-X)活性与男性不育的关系。方法采用聚丙烯酰胺凝胶电泳、全谱酶联染色等方法检测精浆、精子中的LDH-X活性并计算其比值;按照WHO人类精液实验室手册要求对精液进行常规分析。结果83例不育组精浆LDH-X活性(826.0±47.3U/L)与40例生育组LDH-X活性(614.0±22.1U/L)比较有统计学差异(P0.01),同时其精子LDH-X的活性(8.40±3.50mU/106)则小于生育组(15.8±10.8mU/106),差异也具有统计学意义。不育组、生育组精浆/全精子中LDH-X的比值比较差异具有显著性(P0.01)。而两组的精液密度、活动率及精子活力无统计学差异。结论精浆及精子LDH-X活性检测能够反应精子的质量、生育功能以及对受精过程的预期,对选择辅助生育的方法有指导意义。
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| 关 键 词： | 乳酸脱氢酶同工酶 不育 精子 |
Correlation of Activity of Seminal Plasma or Spermatozoa Lactate Dehydrogenase Isoenzyme and Male Infertility |
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| LIU Ya-feng,OU Jian-ping,ZHOU Can-quan,XU Yan-wen,FANG Cong,WANG Qiong,ZHONG Yi-ping,MIAO Ben-yu.. Correlation of Activity of Seminal Plasma or Spermatozoa Lactate Dehydrogenase Isoenzyme and Male Infertility[J]. Chinese Journal of Birth Health & Heredity, 2010, 0(2): 121-122 |
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| Authors: | LIU Ya-feng OU Jian-ping ZHOU Can-quan XU Yan-wen FANG Cong WANG Qiong ZHONG Yi-ping MIAO Ben-yu. |
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| Affiliation: | LIU Ya-feng,OU Jian-ping,ZHOU Can-quan,XU Yan-wen,FANG Cong,WANG Qiong,ZHONG Yi-ping,MIAO Ben-yu.(Center for Reproductive Medcine The First Affiliated Hospital of Sun Yat-Sen Unversituy,Guangzhou 510080,China) |
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| Abstract: | Objective:To study the relationship between activity of seminal plasma or spermatozoa lactate dehydrogenases isoenzyme(LDH-X) and male Infertility. Method: The methods of polyacrylamide gel electrophoresis(PAGE),enzyme -linked staining,desitometric scanning,etc.were used to study LDH-X activity of seminal plasma and spermatozoa obtained from 40 fertile men and 83 infertile men.Semen major parameters were measured and analyzed according to WHO laboratory handbook.Results: The activity of seminal plasma LDH-X... |
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| Keywords: | Lactate dehydrogenase isoenzyme Infertility Spermatozoon |
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