Levels of Schistosoma mansoni Circulating Antigen in Chronic Hepatitis C Patients with Different Stages of Liver Fibrosis |
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| Authors: | Abdelfattah M. Attallah Mohamed El-Far Mohamed M. Omran Khaled Farid Ahmed A. Attallah Dalal Abd-Elaziz |
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| Affiliation: | 1. R &2. D Department, Biotechnology Research Center, New Damietta, Egypt;3. Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt;4. Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt;5. Tropical Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt |
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| Abstract: | The goal of this study was to determine the levels of S. mansoni antigen in different liver fibrosis stages with chronic hepatitis C (CHC) Egyptian patients. A total of 174 CHC patients showing HCV-NS4 antigen and HCV- RNA in their sera were included. S. mansoni antigen was detected in serum using Western blot and ELISA. The levels of interferon-γ (IFN- γ) were determined using ELISA. The 50 kDa S. mansoni antigen discriminated patients infected with S. mansoni from healthy individuals with 0.93 area under curve (AUC), 92% sensitivity, and 97% specificity. The level of S. mansoni antigen (μg/ml) was significantly (P < 0.0001) increased with the progression of liver fibrosis stages (26.9 ± 17.5 in F1, 42.1 ± 25.2 in F2, 49.8 ± 30.3 in F3 and 62.2 ± 26.3 μg/mL in F4 liver cirrhosis), 26.9 ± 17.59 in significant fibrosis (F2–F4); 51.2 ± 27.9 in advanced fibrosis (F3–F4). A significant correlation (r = 0.506; P < 0.0001) was shown between the levels of the S. mansoni antigen and the HCV-NS4 antigen. In conclusion, the presence of S. mansoni antigen in different liver fibrosis stages of CHC patients confirming that concomitant schistosome infection aggravates liver disease. |
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| Keywords: | Hepatitis C virus liver fibrosis schistosoma antigen detection interferon-γ |
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