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Anti-Inflammatory Activity in the Low Molecular Weight Fraction of Commercial Human Serum Albumin (LMWF5A) |
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| Authors: | Gregory W Thomas Leonard T Rael Charles W Mains Denetta Slone Matthew M Carrick Raphael Bar-Or |
| Institution: | 1. Swedish Medical Center, Trauma Research Department, Englewood, Colorado, USA;2. St. Anthony Hospital, Lakewood, Colorado, USA;3. The Medical Center of Plano, Plano, Texas, USA;4. St. Anthony Hospital, Lakewood, Colorado, USA;5. Rocky Vista University, Parker, Colorado, USA;6. The Medical Center of Plano, Plano, Texas, USA |
| Abstract: | The innate immune system is increasingly being recognized as a critical component in osteoarthritis (OA) pathophysiology. An ex vivo immunoassay utilizing human peripheral blood mononuclear cells (PBMC) was developed in order to assess the OA anti-inflammatory properties of the low molecular weight fraction (<5 kDa) of commercial human serum albumin (LMWF5A). PBMC from various donors were pre-incubated with LMWF5A before LPS stimulation. TNFα release was measured by ELISA in supernatants after an overnight incubation. A ≥ 30% decrease in TNFα release was observed. This anti-inflammatory effect is potentially useful in assessing potency of LMWF5A for the treatment of OA. |
| Keywords: | osteoarthritis tumor necrosis factor α peripheral blood mononuclear cells lipopolysaccharide human serum albumin |