| H_2O_2和NO对滑膜成纤维细胞增殖反应的影响 |
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| 引用本文： | 王斌,姚余有,陈敏珠,李华.H_2O_2和NO对滑膜成纤维细胞增殖反应的影响[J].现代免疫学,1998(6). |
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| 作者姓名： | 王斌 姚余有 陈敏珠 李华 |
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| 作者单位： | 安徽医科大学临床药理研究所!合肥230032(王斌，姚余有，陈敏珠)，安徵省卫生干部进修学校!合肥230001(李华) |
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| 基金项目： | 安徽省教委科学基金资助 |
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| 摘 要： | 采用~3H-TdR掺入法和MTT比色法研究过氧化氢(H_2O_2)和一氧化氮(NO)对兔滑膜成纤维细胞(SF)增殖反应的影响。结果表明,在使用亚适SF浓度(5×10~7细胞/L)时,~3H-TdR掺入法和MTT比色法检测结果一致。H_2O_22和亚硝基铁氰化钠(NO供体)在低浓度时促进SF增殖反应,而在高浓度时抑制其增殖反应,提示H_2O_2和NO对SF增殖反应具有双向调节作用。
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| 关 键 词： | 滑膜成纤维细胞 增殖反应 过氧化氢 一氧化氮 |
Effects of H2O2 and NO on the Proliferation of Synovial Fibroblast |
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| Wang Bin,Li Hua,Yau Yuyou,et al..Effects of H2O2 and NO on the Proliferation of Synovial Fibroblast[J].Current Immunology,1998(6). |
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| Authors: | Wang Bin Li Hua Yau Yuyou |
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| Abstract: | Effects of H2O2 and NO on the proliferation of synovial fibroblasts (SF) were studied by using 3H-TdR incorporation and MTT colorimetric assay. The results showed that the proliferation of rabbit SF measured by 3H-TdR incorporation assay was consistent with that by MTT colorimetric assay when suboptimal concentration (5×10~7 cells/L) of SF was used. Both H2O2 and sodium nitroprusside (NO donor) could enhance SF proliferation in lower concentration and suppress its proliferation in higher concentration, suggesting that H2O2 and No possess two-sided regulatory effects on SF proliferation. |
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| Keywords: | synovial fibroblast proliferation hydrogen peroxide nitric oxide |
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