人龈沟液中MMP-2及TIMP-2在牙移动过程中表达的研究

目的 观察龈沟液中基质金属蛋白酶-2（MMP-2）和金属蛋白酶组织抑制因子-2（TIMP-2）的含量随作用于人体牙齿正畸力大小的变化，探讨在牙移动过程中，MMP-2 和TIMP-2的作用与正畸力的关系。 方法 收集患者24例并随机分3组:对照组（0 N），轻力组(0.5 N)，中度力组(1.5 N)。分别牵引上颌尖牙向远中加力0，7，14，21，28 d，收集龈沟液，应用蛋白质印迹法（Western blot）测量龈沟液中MMP-2与TIMP-2含量的变化。 结果 实验组中MMP-2、TIMP-2的含量呈波动性变化，并存在〖JP2〗有时间依赖性，各组间差异有显著性（ P< 0.05）。 结论 轻、中度力下，MMP-2和TIMP-2的表达水平呈显著的波动性变化，并有一定的时间依赖性；MMP-2与TIMP-2参与牙周组织改建过程，二者的动态平衡对牙移动有重要作用。

Study on the functions of MMP-2 and TIMP-2 in human gingival crevicularfluid duringtooth movement

Objective To observe the changes of the contents of matrix metalloproteinase (MMP-2) and tissue inhibitor of metalloproteinase (TIMP-2) in the gingival crevicular fluid (GCF)varying with the differences of orthodontic traction on human teeth,and to explore the relationships between MMP-2 and TIMP-2 functions and the orthodontic traction in the process of tooth movement. Methods Twenty four patients were selected as subjects and divided into three groups randomly:control group (0 N),mild force group (0.5 N),and moderate force group (1.5 N).The traction of canine tooth to distal ends was exerted for 0 days,7 days,14 days and 28 days,and then GCF was collected and the changes of MMP-2 and TIMP-2 contents in GCF were measured by western blot. Results In the experimental groups,MMP-2 and TIMP-2 contents have demonstrated volatility changing feature and were time-dependent; there were significant differences between groups ( P< 0.05). Conclusions Firstly,the expressions of MMP-2 and TIMP-2 demonstrate volatility changing feature,and are time-dependent under mild and moderate forces.Secondly,MMP-2 and TIMP-2 participate in the process of periodontal tissue remodeling and their dynamic balance plays an important role in tooth movement.