加味丹参饮含药血清诱导BMSCs移植IRI大鼠对心肌bFGF的影响

目的:观察加味丹参饮(JWDSY)含药血清诱导的骨髓间充质干细胞(BMSCs),移植于心肌缺血再灌注损伤(IRI)大鼠梗死心肌边缘后,对心肌细胞碱性成纤维细胞生长因子(bFGF)蛋白表达的影响.方法:全骨髓贴壁法培养BMSCs,流式细胞术鉴定细胞表面抗原CD90,CD34,CD45.传至P3代时,用JWDSY含药血清诱导3d.建立大鼠心肌IRI造模,在心肌梗死区边缘移植血清诱导的细胞悬液.3,7,14 d后,取心肌组织用免疫组化法检测心肌细胞bFGF表达.结果:JWDSY血清组bFGF的积分吸光度(IA)在3,7,14d后分别升高至5 758.18±465.17,6 589.61±432.89,8 015.62±366.34;与模型组差异有统计学意义(P＜0.05),且随时间逐渐升高,比较差异有统计学意义(P＜0.05).结论:JWDSY血清诱导的BMSCs移植于IRI大鼠心梗区边缘后,可以改善心肌组织的病理学形态,增加心肌细胞bFGF蛋白表达,提示JWDSY含药血清诱导的BMSCs对IRI大鼠的心肌细胞有一定的保护作用.

Expression of bFGF in Myocardial Cells by Containing Jiawei Danshen Yin Serum in BMSCs Transplanted IRI Rats

Objective:To observe effect of Jiawei Danshen Yin(JWDSY) on the expression of basic fibroblast growth factor (bFGF) in the edge of infarcted myocardium in the rats with ischemia reperfusion injury(IRI) which was transplanted in bone mesenchymal stem cells(BMSCs). Method: BMSCs were cultivated by bone marrow adherent method. The phenotypes of CD90, CD11b, and CD45 were analyzed with flow cytometer. The third generation cells were treated with blank serum or containing JWDSY serum. Rats were modeled as IRI, and then BMSCs were transplanted. In 3 d, 7 d, 14 d, respectively, specimens were taken from each group, myocardial bFGF were detected. Result: IA value of containing JWDSY serum group after 3,7,14 day increased to 5 758.18±465.17,6 589.61±432.89,8 015.62±366.34 respectively. Compared with sham operation groups, the IA value of the rest groups were raised as time gradually, the difference had statistics meanings (P<0.05). Conclusion: The results suggested that BMSCs intervened by containing JWDSY serum could protect the myocardial cells in the IRI rats.