Effect of wortmannin on the suppression of thermotolerance in tsAF8 cells. |
| |
| Authors: | Koichi Shibuya Shoji Kawasaki Masahiro Kuroda Mitsuhiro Takemoto Jun-Ichi Asaumi Yoshio Hiraki |
| |
| Affiliation: | Department of Medical Radioscience, Faculty of Health Sciences, Okayama University Medical School, Okayama 700-8558, Japan. kshibuya@md.okayama-u.ac.jp |
| |
| Abstract: | Phosphatidylinositol 3 kinases (PI-3 kinases) play a crucial role in the inositol phospholipid signaling pathway. The effect of wortmannin, an inhibitor of PI-3 kinases, on the suppression of thermotolerance development was investigated in the temperature-sensitive cell line tsAF8. When wortmannin was added immediately after the preheating, the thermotolerance was suppressed, with this suppression being dose-dependent from 0.01 to 0.1 microM, but not dose-dependent from 0.1 to 20 microM. Our results suggest that PI-3 kinases might play an important role in thermotolerance development in tsAF8 cells. |
| |
| Keywords: | |
|
|
