槲皮素对人肝癌SMMC-7721细胞生长的抑制及细胞凋亡的诱导作用

目的探讨槲皮素体外对人肝癌SMMC-7721细胞的生长抑制作用及诱导细胞凋亡的作用机制。方法以10、30、60、100#mol／L槲皮素作用于体外培养的SMMC-7721细胞，MTT法检测细胞生长抑制率，Annexi—V／PI双染流式细胞仪检测细胞凋亡情况，免疫组化染色法检测bcl-2、bax蛋白表达的变化。结果30、60、100umol／L槲皮素可显著地抑制SMMC-7721细胞的生长（P〈0．01），诱导细胞发生凋亡（P〈0．01），并呈现量墩和时-效关系；槲皮素作用48h后，免疫组化染色法检测显示bcl-2蛋白表达降低和bax蛋白表达上调。结论槲皮素体外通过引起人肝癌SMMC-7721细胞bcl-2蛋白表达下调和bax蛋白表达上调诱导细胞发生凋亡，抑制细胞生长，槲皮素可能成为一种潜在的抗肝癌药物。

Mechanisms of growth inhibition and apoptosis inducement of quercetin on human hepatocellular carcinoma SMMC-7721 cells

Objective To study the mechanism of cell growth inhibition and apoptosis inducement of quercetin on human hepatocellular carcinoma SMMC-7721 cells. Methods SMMC-7721 cells in cultural medium in vitro were given 10, 30, 60 and 100 umol/L quercetin. The inhibitory rate of cells was measured by MTT assay. The cell apoptotic rate was detected by flow cytometry with Annexi-V/PI staining. The expression of bcl-2 and bax in SMMC-7721 cells were detected by cell immuneohistochemistry. Results 30, 60 and 100 umol/L qucertin could inhibit the growth of SMMC-7721 cells and cause cell apoptosis significantly （P 〈0.01）. The suppression was dependent both on the time and on the dosage. 48 hours after the treatment of qucertin, the detection of cell immuneohistochemistry showed the bcl-2 protein down-regulation and bax protein up-regulation （P 〈0.05）. Conclusion Qucertin can inhibit the human hepatocellular carcinoma SMMC-7721 cells growth by inducing the cell apoptosis in vitro through down-regulation of bcl-2 expression and up-regulation of bax in protein levels. The results indicate that quercetin may be an important and potential anti-hepatocellular carcinoma reagent.