| 基于邻位连接技术（PLA）的生物检测新策略 |
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| 引用本文： | 郑磊,尹小毛,王前. 基于邻位连接技术（PLA）的生物检测新策略[J]. 分子诊断与治疗杂志, 2012, 4(4): 217-221 |
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| 作者姓名： | 郑磊 尹小毛 王前 |
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| 作者单位： | 南方医科大学南方医院检验医学科,广东,广州510515 |
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| 基金项目： | 国家质检总局科技计划，广东省自然科学基金，广东省科技计划 |
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| 摘 要： | 邻位连接技术（PLA）是近年来发展起来的一种蛋白质检测方法。该技术的核心理念来源于适体技术、连接酶技术和Taqman探针技术，其主要形式包括均相、固相和原位3种。PLA具有特异、灵敏、快速、高通量和多功能等优点，不但能够检测蛋白质、亚细胞结构和细胞等生物样本，还能检测大分子间的相互作用，现已逐步被应用于医学和生物学等众多领域。
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| 关 键 词： | 邻位连接技术 蛋白质 适体 连接酶链反应 实时PCR |
Biological detection based on proximity ligation assay |
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| ZHENG Lei , YIN Xiaomao , WANG Qian. Biological detection based on proximity ligation assay[J]. Journal of Molecular Diagnosis and Therapy, 2012, 4(4): 217-221 |
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| Authors: | ZHENG Lei YIN Xiaomao WANG Qian |
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| Affiliation: | (Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangdong, Guangzhou 510515, China) |
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| Abstract: | Proximity ligation assay（PLA） is a newly developed technology for protein detection, which originated from aptamer technology, ligation chain reaction and Taqman probe real-time PCR. There are mainly three types of PLA, namely homogenous PLA, solid-phase PLA and in situ PLA. This technology is specific, sensitive, rapid, high-throughput and multiftmctional. PLA can be used for detection of biospecimens and analysis of interaction between macromolecules, so it has been extensively applied in many fields, such as medicine and biology. |
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| Keywords: | Proximity ligation assay Protein Aptamer Ligation chain reaction Rt-PCR |
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