血管紧张素转换酶和血管紧张素原基因多态性及其与青海妊娠高血压疾病的相关性

目的探讨血管紧张素转换酶(ACE)和血管紧张素原(AGT)基因表达、基因多态性与青海孕产妇妊娠高血压疾病的相关性。方法选择210例妊娠高血压患者(HDCP组)和220例正常孕妇(CK组),运用限制性内切酶片段长度多态性聚合酶链反应(PCR-RFLP)方法检测AGT M235T、ACE I/D基因多态性。结果CK组ACE基因DD、ID、Ⅱ所占比例分别为28.18%、47.73%、24.09%,HDCP组分别为33.81%、51.90%、14.29%(P<0.05,故两组的ACE基因分布有差异),HDCP组和对照组ACE I/D多态性等位基因I和D频率分布有差异(P<0.05),HDCP组D等位基因频率高于对照组(χ^2=5.188,P<0.05),ACE基因型分布符合Hardy-Weinberg遗传平衡(χ^2=0.423,df=2,查表χ^2界值表,P>0.05,达到遗传平衡);CK组AGT基因MM、MT、TT所占比例分别为24.09%、43.64%、32.27%,HDCP组分别为15.71%、42.86%、41.43%(P<0.05,故两组的AGT基因分布差异有统计学意义),HDCP组和对照组AGT M235T多态性等位基因M和T频率分布有差异性(P<0.05),HDCP组T等位基因频率高于对照组(χ^2=6.796,P<0.05),AGT基因型分布符合Hardy-Weinberg遗传平衡(χ^2=3.242,df=2,查表χ^2界值表,P>0.05,达到遗传平衡)。结论ACE I/D多态性和AGT M235T多态性与青海省汉族妊娠期高血压疾病有关,D等位基因和T等位基因可能是妊娠高血压疾病的易感基因。

Association between angiotensin converting enzyme and angiotensinogen gene polymorphisms and pregnancy-induced hypertension in Qinghai#br#

Objective To investigate the relationship between angiotensin converting enzyme (ACE) and angiotensinogen (AGT) gene expression, gene polymorphism and pregnancy-induced hypertension in Qinghai.Methods A total of 210 pregnant hypertensive patients (HDCP group) and 220 normal pregnant women (CK group) were enrolled. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect AGT M235T and ACE I/D gene polymorphism. Results The proportions of ACE gene DD, ID, and Ⅱ in CK group were 28.15%, 47.73%, and 24.09%, respectively. The HDCP group was 33.81%, 51.90%, and 14.29%, respectively (P<0.05). The frequency distribution of ACE I/D polymorphic alleles I and D was different between HDCP group and CK group(P<0.05). D allele frequency was higher in HDCP group than in CK group (χ2=5.188, P<0.05). ACE genotype distribution was accorded with Hardy-Weinberg genetic balance(χ2=0.423, df=2, check table χ2 boundary value table, P>0.05, reach genetic balance). The proportions of MM, MT and TT in CK group was 24.09%, 43.64% and 32.27%, respectively. The HDCP group was 15.71%, 42.86%, and 41.43% respectively (P<0.05). HDCP group and CK group AGT M235T polymorphic alleles M and T frequency distribution were different (P<0.05). HDCP group T allele frequency was higher than the CK group(χ2=6.796, P<0.05). AGT gene were genotype distribution accorded with Hardy-Weinberg genetic balance (χ2=3.242, df=2, check table χ2 boundary value table, P>0.05, reaching genetic balance).Conclusion The ACE I/D polymorphism and AGT M235T polymorphism are associated with hypertensive disorder of pregnancy in Han nationality in Qinghai Province. D allele and T allele may be susceptible genes of pregnancy-induced hypertension.