Development and validation of analytical method for the estimation of lamivudine in rabbit plasma |
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Authors: | Akhilesh Vikram Singh Lila K Nath Nihar R Pani |
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Institution: | 1. Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India;2. Gayatri College of Pharmacy, Sambalpur, Orissa, India |
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Abstract: | Lamivudine has been widely used in the treatment of HIV disease. A reliable, sensitive reversed phase high performance liquid chromatography (RP-HPLC) method was developed and validated for lamivudine in rabbit plasma. The method was developed on Hypersil BDS C-18 column (250 mm×4.6 mm, 5 μm) using a mobile phase of 0.25% Triethylamine buffer (pH 3.0): acetonitrile (70:30, v/v). The efficient was monitored by UV detector at 256 nm. The total run time was 15 min with a flow rate of 1.0 mL/min. Calibration curve was linear over the concentration range of 25–2000 ng/mL. The retention times of lamivudine and internal standard (Nelfinavir) were 8.78 min and 10.86 min, respectively. The developed RP-HPLC method can be successfully applied for the quantitative pharmacokinetic parameters determination of lamivudine in rabbit model. |
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