| 急性淋巴细胞白血病Rb基因的分析 |
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| 引用本文： | 舒青 曾瑞萍. 急性淋巴细胞白血病Rb基因的分析[J]. 医学争鸣, 1996, 17(3): 172-174 |
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| 作者姓名： | 舒青 曾瑞萍 |
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| 作者单位： | [1]第四军医大学生物学教研室 [2]中山医科大学遗传教研室 |
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| 摘 要： | 应用限制性内切酶片段长度多态性分析了３２例原发急性淋巴细胞白血病Ｒｂ基因的等位基因变化，采用＾３２Ｐ－标记的ＲｂＣｄｎａ３．８ｋｂ探针，结果在８例患者中发现Ｒｂ基因结构异常。其中４例出现新的３．１ｋｂ和２．３ｋｂ片段，选用Ｒｂ基因外显子１８，１９，２１，２２，２７五对引物，对已发现Ｒｂ基因异常的６例患者进行了分析，发现１例在外显子１８和２１无扩增产物。
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| 关 键 词： | Rb基因 急性 白血病 基因缺失 |
Analysis of Rb gene in primary acute lymphoid leukemia |
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| Shu Qing ,Zeng Ruiping ,Liu Suying ,Gao Qing ,Du Chuanshu. Analysis of Rb gene in primary acute lymphoid leukemia[J]. Negative, 1996, 17(3): 172-174 |
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| Authors: | Shu Qing Zeng Ruiping Liu Suying Gao Qing Du Chuanshu |
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| Affiliation: | Shu Qing 1,Zeng Ruiping 2,Liu Suying 1,Gao Qing 1,Du Chuanshu 2 1Department of Biology,Fourth Military Medical University,Xi'an 710033 2Department of Medical Genetics,Sun Yat sen University of Medical Science |
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| Abstract: | The structure of the Rb gene in 32 cases of acute lymphoid leukemia (ALL) were studied by Southern blotting using 32 P labeled Rb cDNA 3.8 kb probe. The structural abnormal alterations of Rb gene in DNA from 8 cases of ALL were found, an incidence of 25%. Two novel fragment (3.1 kb, 2.3 kb) were observed in five of 8 cases. We used five pair of primers of exon 18, 19, 21, 22, 27 to amplify Rb gene from 6 cases of ALL with abnormal Rb gene. Products of exon 18 and 21 were not found in one case. The results implied that abnormalities of Rb gene might be associated the progression or initiation of ALL. |
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| Keywords: | Rb gene acute lymphoid leukemia gene deletion |
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