荧光定量PCR检测乙型肝炎病毒DNA的临床价值

目的：探讨荧光定量PCR（ FQ-PCR）检测乙型肝炎病毒（ HBV）-DNA的临床意义。方法：采用FQ-PCR检测265例乙型肝炎（乙肝）患者和150名健康体检者的血清HBV-DNA,并将结果与酶联免疫吸附试验测定的乙肝血清标志物进行比较。结果：不同血清标志物模式的各组均可检出 HBV-DNA 阳性,其中 HBsAg、HBeAg、HBcAb 阳性组和 HBsAg、HBeAg 阳性组的HBV-DNA阳性率和病毒拷贝数均显著高于其他血清标志物模式组和对照组（P〈0.01）。 HBV所致不同疾病类型的HBV-DNA阳性率差异均无统计学意义（P〉0.05）,但急性乙肝病毒拷贝数均明显高于其他疾病（P〈0.01）。结论：FQ-PCR检测HBV-DNA可以直接反映是否存在HBV感染,判断病毒的复制能力和传染性,指导临床用药和观察疗效,具有重要的临床意义。

Clinical value of fluorescent quatititive polymerase chain reaction in the detection of hepatitis B Virus-DNA

Objective：To investigate the clinical value of fluorescent quatititive polymerase chain reaction（ FQ-PCR） in the detection of hepatitis B Virus（ HBV）-DNA. Methods：The positive rate and copies of HBV-DNA in 265 HBV patients and 150 healthy people were detected using FQ-PCR, which were compared with the serological markers of hepatitis B detected by enzyme linked immunosorbent assay. Results：The HBV-DNA was detected in all cases with different serological markers,the positive rate and copies of HBV-DNA in HBsAg,HBeAg and HBcAb（ ＋） group and HBsAg and HBeAg（ ＋） group were higher than those in other groups （P〈0. 01）. The difference of the positive rate of HBV-DNA in different diseases types caused by HBV was not statistically significant （P〉0. 05）,the copies of HBV in acute hepatitis patients were significantly higher than that in other groups（P〈0. 01）. Conclusions：The HBV-DNA detected by FQ-PCR can directly reflect the HBV infection, judge the ability of replication and infection, guide the clinical medication and observe curative effects.