| Runx2/cbfa1在人牙胚发育过程中的表达 |
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| 引用本文： | 段学静,杨丕山,李纾,潘克清,谢瑞阅,马伯龙.Runx2/cbfa1在人牙胚发育过程中的表达[J].临床口腔医学杂志,2005(10). |
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| 作者姓名： | 段学静 杨丕山 李纾 潘克清 谢瑞阅 马伯龙 |
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| 作者单位： | 山东大学口腔医学院 山东济南250012
(段学静，杨丕山，李纾，潘克清，谢瑞阅)，山东大学口腔医学院 山东济南250012(马伯龙) |
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| 基金项目： | 国家自然科学基金资助课题(30371537)山东省优秀中青年科学家奖励基金(02BS096) |
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| 摘 要： | 观察核心因子Runx2/cbfa1在人牙胚发育过程中的表达,探讨cbfa1在牙齿发育过程中的作用。方法:制备人牙胚发育各期标本,免疫荧光法观察cbfa1在人牙胚发育过程中的表达情况。结果:在牙胚发育的不同时期均可见不同的时空表达模式。蕾状期表达比较广泛;帽状期内外釉上皮细胞和牙囊中呈强阳性表达,中间层也为强阳性,牙乳头为弱阳性;钟状早期,前成牙本质细胞处有强阳性表达;钟状中后期,成釉细胞为强阳性,成牙本质细胞为阴性,而牙囊部位仍为强阳性。结论:Runx2/cbfa1转录因子在人牙胚整个发育过程中的表达状况,提示该因子可能在牙胚各期的发育起一定作用,参与了各种成牙相关细胞的分化和硬组织的形成。
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| 关 键 词： | 核心结合因子a1 牙胚发育 免疫荧光 |
The expressions of Runx2/cbfa1 during human tooth germ development |
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| DUAN Xue-jing,YANG Pei-shan,LI Shu,PAN Ke-qing,XIE Rui-yue,MA Bo-long.College of Stomatology,Shandong University,Jinan ,China.The expressions of Runx2/cbfa1 during human tooth germ development[J].Journal of Clinical Stomatology,2005(10). |
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| Authors: | DUAN Xue-jing YANG Pei-shan LI Shu PAN Ke-qing XIE Rui-yue MA Bo-longCollege of Stomatology Shandong University Jinan China |
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| Institution: | DUAN Xue-jing,YANG Pei-shan,LI Shu,PAN Ke-qing,XIE Rui-yue,MA Bo-long.College of Stomatology,Shandong University,Jinan 250012,China |
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| Abstract: | Objective:To study the role of Runx2/cbfa1 in human tooth development.Method:The specimen of the human's tooth germ in different stages were made to observe the expressions of cbfa1 during tooth germ development by immunofluorescence method.Result:Cbfa1 had its own specific temporal-spatial pattern during tooth germ development.In bud stage,the factor expressed widely;in cap stage,the inner and outer enamel epithelium showed strong positive expressions,as well as the medium layer.While the dental papilla showed weak expression;in the early bell stage,preodontoblast expressed positively;in the medium and late bell stage,adamantoblast was positive while odontoblast was negetive.Conclusion:The expressions of Runx2/cbfa1 during tooth germ development indicates that this factor possibly plays a role in every stage of tooth germ development,participating the differentiation of kinds of cells and the hard tissue formation. |
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| Keywords: | Runx2/cbfa1 tooth development immunofluorescence |
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