海南广藿香试管内芽分化与愈伤组织诱导研究

目的选择海南广藿香的最佳芽分化和愈伤组织诱导培养基。方法利用海南广藿香的叶柄、茎尖和带节茎段进行组织培养芽分化试验，并对上述材料进行愈伤组织诱导培养基选择试验。结果在芽分化培养基中，MS＋3-吲哚丁酸（IBA）1．0mg／L＋6-苄基腺嘌呤（6-BA）0．6mg／L培养基能够较好地诱导茎尖和带节茎端长出新芽，诱导率分别为94．3％和94．6％；在愈伤组织诱导培养基中，MS＋萘乙酸（NAA）0．1mg／L＋6-BA2．0mg／L培养基能较好地诱导海南广藿香叶柄和带节茎段产生愈伤组织，诱导率分别为80．0％和100％。结论海南广藿香的最佳芽分化和愈伤组织诱导培养基分别为Ms＋IBA1．0mg／L＋6-BA0．6mg／L和Ms＋NAA0．1mg／L＋6-BA2．0mg／L。

Study on Bud Differentiation and Callus Induction of Hainan Pachouli in Vitro

Objective To select the best culture medium of bud differentiation and induction for callus of Hainan patchouli. Methods To carry the petiole, stem tips and stem segment of Hainan patchouli on the difference tissue culture medium in vitro to develop the experiments of bud differentiation and callus induction. Results In experiment of bud differentiation, the culture medium MS ＋ 3 - IBA 1.0 mg/L ＋ 6-BA 0.6mg/L could induce the stem tips and the stem segment produced new bud well, the induction rate are 94.3% and 94. 6%;the petiole and the stem segment of Hainan patchouli can be induced the callus well in the culture medium MS ＋ NAA 0. 1 mg/L ＋6- BA 2.0 mg/L, the induction rates were 80.0% and 100. 0% respectively. Conclusion The best culture medium of bud differentiation and induction for callus of Hainan patchouli is MS ＋ IBA 1.0 mg/L ＋ 6 - BA 0. 6 mg/L and MS ＋ NAA 0. 1 mg/L ＋ 6 - BA 2.0 mg/L,respectively.