D-氨基半乳糖致小鼠、大鼠肝损伤模型的实验性研究

目的筛选D-氨基半乳糖（D—Gal）造模的最佳剂量，比较D—Gal造成的大、小鼠急性肝损伤模型的稳定性，为保肝新药的筛选提供合适的实验动物模型和理论依据。方法采用60只昆明种小鼠、70只SD大鼠，以D—Gal作为肝损伤试剂，建成急性肝损伤动物模型，以血液生化、肝功能、肝组织学的改变为观察指标，筛选大、小鼠最佳用药剂量，并观察D—Gal所致大鼠肝损伤的动态变化。结果本实验条件下，D—Gal500mg／kg为大鼠的最适剂量，此剂量建立的肝损伤模型肝组织形态变化显著且死亡率低。结论与小鼠动物模型相比较，D—Gal500mg／kg大鼠急性肝损伤模型更适用于保肝新药的筛选与评价。

The making of a model of hepatic injury induced with D-galactosamine in rats

Objective To optimize the dosage of D-galactosamine(D-Gal) to make a mouse/rat model of hepatic injury as a tool for the development of hepatic drugs.Methods The model was induced with different doses of D-Gal ip in 70 rats and 60 mice.The levels of ALT and AST were determined by using automatic biochemical analyzer at 12,24,48 and 72 h.Liver specimens were processed for pathological examination.Results Comprehensive evaluation of the results showed that the use of D-Gal 500 mg/kg ip in rats could induce stable models with low mortality.Conclusion The hepatic injury model induced with D-Gal 500 mg/kg ip in rats,better than that induced in mice,is recommended for hepatic pharmaceutical studies.