磷酸肌酸钠对大鼠肢体缺血再灌注心肌损伤的影响

目的研究磷酸肌酸钠对肢体缺血再灌注后心肌损伤有无保护作用及其机制。方法选取SD大鼠24只,将其随机分为三组:①空白对照组(C组);②肢体缺血再灌注组(LIR组);③磷酸肌酸钠组(P组),每组8只。分别检测血清肌钙蛋白I(c-TnI)、磷酸肌酸激酶同工酶(CK-MB),心肌组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、钙调蛋白(CaM)的含量,并且观察各组心肌组织的病理变化。结果 LIR组与C组相比,血清c-TnI、CK-MB及心肌组织中MDA、CaM的含量都显著增高,而SOD含量明显下降,心肌组织有明显的病理变化;P组与LIR组相比,SOD活性相对升高,c-TnI、CK-MB、MDA、CaM含量相对降低,病理变化有所改善。结论肢体缺血再灌注可诱发心肌组织的损伤;外源性磷酸肌酸钠可减少肢体缺血再灌注心肌组织内氧自由基的产生,维持细胞内钙平衡,起到保护心肌的作用。

Effects of creatine phosphate on myocardium injury of limb ischemia reperfusion in rats

Objective To investigate effects and mechanisms of creatine phosphate on myocardium injury induced by limb ischemia reperfusion in rats. Methods Twenty-four rats were randomly divided into 3 groups： sham-operated con- trol （C） group,limb ischemia reperfusion （LIR） group and creatine phosphate （P） group. The level of c-TnI,CK-MB in plasma and MDA, SOD, CaM in myocardial tissue were measured in each group. The cardiac histopathology changes in experimental rats were observed. Results In LIR group,the level of c-TnI,CK-MB in plasma and MDA,CaM in my- ocardial tissue were significantly increase and the contents of SOD was decreased compared with C group. And under light microscope,myocardial impairment was worst compared with the other two groups. In P group, the activity of SOD was significantly increased and the contents of MDA,CaM,c-TnI,CK-MB was decreased compared with LIR group. The cardiac histopathologic changes was better than P group. Conclusion Myocardium injury can be induced by limb ischemia-reperfusion. Creatine phosphate can protect the myocardium from limb ischemia-reperfusion,and the protec- tive effects may be related to balance of calcium and metabolism of oxygen free radicals.