低氧预处理对大鼠脑缺血再灌注后梗死体积和血脑屏障通透性的影响

目的　探讨低氧预处理对大鼠脑缺血再灌注损伤的保护作用。方法　将SD大鼠分为 3组 ,即假手术组、缺血再灌注组、缺氧预处理 +缺血再灌注组。连续吸入 8%O2 +92 %N2 3h作缺氧预处理 ,12h后再经插线左大脑中动脉栓塞 (MCAO)制作缺血再灌注模型 ,到相应时间点后观察缺氧预处理对MCAO大鼠的行为、脑含水量、血脑屏障通透性和脑梗死体积的影响。结果与缺血再灌注组相比 ,缺氧预处理组大鼠的行为明显改善 ,脑伊文思蓝 (EB)含量、脑含水量 (P <0 0 5 ) ,脑梗死体积缩小。结论　低氧预处理降低缺血再灌注脑组织血脑屏障通透性 ,抑制脑水肿 ,缩小梗死体积 ,对缺血再灌注损伤具有保护作用

Effect of hypoxic preconditioning to blood-brain-barrier and cerebral infarction size after transient focal cerebral ischemia in rats

Objective To observe the role of hypoxic preconditioning to blood-brain-barrier and cerebral infarction size after transient focal cerebral ischemia in rats Methods SD rats were divided into three groups randomly, including sham-operated group, hypoxic preconditioning group and hypoxic preconditioning plus transient cerebral ischemia group Following 3h of 8%oxygen+92%nitrogen, the SD rats were made into transient cerebral ischemic models by occluding left middle cerebral artery At each time-point, neurological impairment, brain water content, permeability of BBB, and cerebral infarction size were observed Results Compared to transient cerebral ischemia group, neurological impairment in hypoxic preconditioning plus transient cerebral ischemia group ameliorated (p<0 05), brain water content, permeability of BBB and cerebral infarction volume in hypoxic preconditioning plus transient cerebral ischemia group descended (p<0 05) Conclusion Hypoxic preconditioning plays an important role of cerebral protection in focal cerebral ischemia in rats