Spectrin expression in neuroblastoma cells |
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| Authors: | Steven R. Goodman Ian S. Zagon Diane B. Coleman Patricia J. McLaughlin |
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| Affiliation: | 1. Grup MAiMA, SGR Mineralogia Aplicada, Geoquímica i Geomicrobiologia, Departament de Mineralogia, Petrologia i Geologia Aplicada, Facultat de Ciències de la Terra, Universitat de Barcelona (UB), Barcelona, Spain;2. Sustainability Department, Fundació CTM Centre Tecnològic, Spain;3. Sustainability Area, Eurecat, Centre Tecnològic de Catalunya, Spain;4. Amphos 21 Consulting SL, Spain;5. Catalana de Perforacions, Spain;6. Serra Húnter Fellow, Generalitat de Catalunya, Spain |
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| Abstract: | Mouse neuroblastoma cells express a spectrin-related molecule containing 240 kDal (kiloDalton) and 235 kDal subunits in a 1:1 ratio. The 240 kDal and 235 kDal subunits are nearly identical to the alpha and beta subunits respectively of brain spectrin by two dimensional chymotryptic peptide mapping analysis. The neuroblastoma cells do not express a measureable quantity of a red blood cell (rbc)-type spectrin molecule. Neuroblastoma spectrin has been localized throughout the cell body, and neurites of these cells by indirect immunofluorescence studies. As neuroblastoma cells are homogeneous, neuron-like, available in large quantity, and synthesize a single variant of spectrin which is closely related to brain spectrin(240/235), it is the best available model system for the study of the synthesis, assembly and turnover of a neuronal spectrin subtype. |
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| Keywords: | Spectrin subtypes Neuroblastoma cells Immunoprecipitation Peptide mapping Indirect immunofluorescence |
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