| 重组P_(53)基因在HepG-2中的表达及作用 |
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| 引用本文： | 任碧轩 李仁 冯莉 唐恩洁,杨晓红 赵明才 杨健 张紫福 张艳艳 魏祥云.重组P_(53)基因在HepG-2中的表达及作用[J].川北医学院学报,1999,14(2). |
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| 作者姓名： | 任碧轩 李仁 冯莉 唐恩洁 杨晓红 赵明才 杨健 张紫福 张艳艳 魏祥云 |
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| 作者单位： | ：任碧轩 李 仁 冯 莉 唐恩洁(川北医学院分子生物学研究室 南充 637000);杨晓红 赵明才 杨健(微生物学教研室) |
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| 摘 要： | 为进一步探索正常Ｐ５３ 对肿瘤细胞增殖的抑制作用，本文用磷酸钙 ＤＮＡ共沉淀法，将本室构建的ＰＸＴ１ Ｐ５３ 真核表达细胞转移至ＨｅｐＧ ２ 肝癌细胞系内，经斑点杂交技术和间接免疫荧光技术证实，外源性Ｐ５３ 基因已在ＨｅｐＧ ２ 细胞中稳定表达，导入的Ｐ５３基因亦能抑制ＨｅｐＧ ２ 肝癌细胞系增殖并诱导其凋亡。实验结果提示正常Ｐ５３ 基因可成为治疗不同肿瘤的重要靶分子之一。
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| 关 键 词： | 重组P_(53)基因 HepG-2细胞 表达 |
| 文章编号： | 1005-3697(1999)02-0001-04 |
| 修稿时间： | 1999-03-16 |
Expression in the HepG 2 and Effect on HepG 2 of the Recombinant P 53 Gene |
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| Ren Bi Xuan, Li Ren, Tang EnJie et
al
North Sichuan Medical College.Expression in the HepG 2 and Effect on HepG 2 of the Recombinant P 53 Gene[J].Journal of North Sichuan Medical College,1999,14(2). |
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| Authors: | Ren Bi Xuan Li Ren Tang EnJie et
al
North Sichuan Medical College |
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| Abstract: | In order to investigate further(normal P 53 gene inhibits the proliferation of Tumor, the recombinant P 53 gene was transferred into the HepG 2 cell line using the calcium phosphate DNA Coprecipitation method. The Dig P 53 probe and anti P 53 were used to analyze the expression in HepG 2 by Dot blot hybridization and indirect immunofluorecent labeling technique. It was confirmed the HepG 2 could express the recombinant P 53 gene. Meanwhile, it has been observed that the transferred P 53 gene could inhibit the proliferation of HepG 2 and induce the apoptosis of HepG 2. The experimental results suggested normol P 53 gene may be an important therapeutic targeting used to treat the different tumors. |
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| Keywords: | recombinant P 53 gene HepG 2 cell line |
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